Dendritic cell functionin vitro: antigen uptake, maturation and adhesion. (A) Immature BMDCs were incubated for different periods of time with DQ-OVA, and the mean fluorescence intensity (as quantification of antigen uptake) was measured by FACS (mean ± SEM, n = 6). (B) BMDCs were matured in the presence of LPS for 24 h. Control, immature BMDCs. The upregulation of CD86 maturation marker expression is shown as mean fluorescence intensity (MFI; mean + SEM, n = 3). (C) LPS-matured BMDCs were plated onto ICAM-1-coated plates and fibronectin-coated plates. After incubation for 2 h, the numbers of adherent cells were counted (mean + SEM, n = 5).
Togni et al. Cell Communication and Signaling 2012 10:14 doi:10.1186/1478-811X-10-14