Figure 5.

Cytokine production and signaling after LPS stimulation. (A) BMDCs from wild-type animals (WT) and ADAP-deficient animals (ADAP) were stimulated for 24 h in the presence of LPS, and the production of cytokines TNF-∝ and IL-6 was measured in the supernatants (mean + SEM, n = 7 to 9). BMDCs were stimulated with LPS for the indicated time, lysed, and then immunoblotted with antibodies to investigate NF-κB signaling (B), and the MAP kinase signaling pathway (C). One representative of three independent experiments is shown.