Figure 1.

Scheme of the mouseNfatc1gene, its inducible promoter P1 (above) and the inducible short isoform NFATc1/αA (below) (modified according to refs.[[35],[44],[49],[50],[96]]). In the promoter sequence, vertical dashes indicate CpG residues. Binding sites for transcription factors are shown as filled circles (for Sp1/Sp3 binding) or as boxes for the binding of various other factors. The murine Nfatc1 gene spans approximately 110 kb DNA and is divided into 11 exons. Its expression is directed by two promoters, P1 and P2, and two poly A addition sites, pA1 and pA2. For the generation of NFATc1/αA RNA (encoding the α-peptide), the induction of P1 promoter results in the transcription of exon 1, splicing to exon 3 and poly A addition at the poly A site pA1. RNAs encoding the β-isoforms (with the β-peptide encoded by exon 2) are directed by promoter 2. The sequence of the N-terminal α–peptide from NFATc1/αA is given below. For NFATc1/αA, the Rel Similarity Domain, RSD, and transactivation domain, TAD-A, are indicated. In analogy to other NFATc factors (see Refs. [1-3]), two sites A and B for the binding of calcineurin, a nuclear localization signal, NLS, and several Ser-rich motifs that are phosphorylated, are also indicated. Below NFATc1/αA, the aa sequence of N-terminal α-peptide is shown in which all Pro and Ser/Thr residues are underlined.