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Open Access Research

Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment

Lars Wiechert1, Julia Németh1, Tobias Pusterla1, Christine Bauer1, Aurora De Ponti1, Sandra Manthey2, Silke Marhenke3, Arndt Vogel3, Ursula Klingmüller2, Jochen Hess45* and Peter Angel1

Author Affiliations

1 Division of Signal Transduction and Growth Control, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Heidelberg, Germany

2 Division of Systems Biology of Signal Transduction, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Heidelberg, Germany

3 Department of Hepatology, Medical School Hannover, Hannover, Germany

4 Junior Group Molecular Mechanisms of Head and Neck Tumors, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Heidelberg, Germany

5 Department of Otolaryngology, Head and Neck Surgery, University Hospital Heidelberg, Heidelberg, Germany

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Cell Communication and Signaling 2012, 10:40  doi:10.1186/1478-811X-10-40

Published: 15 December 2012

Abstract

Background

Calprotectin consists of the Ca2+-binding proteins S100a8 and S100a9 that are induced in epithelial cells in response to tissue damage and infection. Both proteins are also secreted by activated innate immune cells and numerous studies demonstrate their crucial role in pathological conditions of acute and chronic inflammation.

Results

Here, we established a conditional mouse model with simultaneous S100a8 and S100a9 transgene expression in hepatocytes (TgS100a8a9hep) under the control of doxycycline to unravel the role of epithelial-derived Calprotectin on tissue homeostasis and inflammation. TgS100a8a9hep mice displayed a significant enrichment of neutrophils in peripheral blood and tissues with high blood content. Interestingly, Cxcl1 transcription was significantly induced in the liver of TgS100a8a9hep mice and primary hepatocytes derived thereof as compared to Control mice, accompanied by an increase of Cxcl1 serum levels. However, expression of other chemokines with a known function in neutrophil mobilization from the bone marrow, e.g. Csf3 and Cxcl2, was not altered. Doxycycline treatment of TgS100a8a9hep mice reduced Cxcl1 expression in the liver and resulted in normal numbers of neutrophils.

Conclusion

In summary, our data demonstrate for the first time that hepatocyte-specific S100a8 and S100a9 expression induces a systemic mobilization of neutrophils by a specific activation of Cxcl1 transcription in the liver.

Keywords:
Calgranulins; Calprotectin; Hepatocytes; Neutrophils; Chemokines; Immune cell mobilization