Figure 2.

Effects of actin dynamics and the cofactor MAL on SRF activity. (A) p3D.A-Luc activity of Jurkat T cells transiently transfected with expression plasmids coding for actin wt, actinR62D, MAL and MALΔNΔB1 alone or in combination with a Tip expression construct. (B) Representative expression controls carried out by immunoblot analysis with whole cell lysates 48 h post transfection. Detection of Hsp90α/β served as a loading control. (C) p3D.A-Luc activity of vector- and Tip-transfected cells treated with Latrunculin B (1 μM) and Cytochalasin D (1 μM) for 24 h. The graphs in (A) and (C) display the mean values and standard deviation of three independent experiments. Statistical significance, p > 0.05 (ns); p < 0.05 (*); p < 0.01 (**)