Table 1 |
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|
PCR primers used for the detection of IL-8 and of T-type Ca++-channel α1 subunits. |
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| Primer sequence |
Annealing temperature |
PCR product |
Ref. |
|
|
|
||||
| IL-8 |
5'-CCACCCATGGCAAATCCATGGC-3' 5'-TCTCAGCCCTCTTCAAAAACTTCTC-3' |
65°C |
289 |
[31] |
| α1G |
5'-CCTGGACTTCTTCACGATGT-3' 5'-CCAGGTCTGCTGGGTCAGAG-3' |
63°C |
395 |
[32] |
| α1H |
5'-TGTTCGTGACGGACTCGAATT-3' 5'-AGTGCACAGAGGCAACGGA-3' |
63°C |
436 |
[32] |
| α1I |
5'-TTCCCCTACACCGGAACGG-3' 5'-TAGTAACGGTTCCAGTTGA-3' |
50°C |
227 |
[32] |
| HPRT |
5'-CCTGCTGGATTACATTAAAGCACTG-3' 5'-CTTCGTGGGGTCCTTTTCACCAGC-3' |
65°C |
370 |
Genbank n. M26434 |
| β-actin |
5'-GGAAATAGGGGTTAGCAC-3' 5'-CTCATGTGCGCCTACTTA-3' |
56°C |
929 |
Genbank n. AJ005353 |
Marino et al. Cell Communication and Signaling 2004 2:6 doi:10.1186/1478-811X-2-6 |
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