Figure 2.

Reactivity of affinity purified anti-K19M-AF antibodies in ELISA. Affinity chromatography fractions were tested in wells coated with: a) GST 1 μg/ml (hatched columns); b) GST-CCN3 1 μg/ml (blank columns); c) K19M peptide 1 μg/ml (black columns). Column fractions F1 to F3 contain the flow through unbound proteins while fractions F4 to F8 contain antibody bound to K19M peptide that eluted with glycine buffer pH 2.8.