Figure 4.

Detection of CCN3 expression on cultured cell lines by cell-ELISA. Cells from H295R line or glioblastoma G59 cells were treated with 1% bovine serum albumin in PBS, pH 7.4 (blank columns) or K19M-AF diluted 1/500 in 1%BSA (hatched columns). Treatment of cells: A – G59 non-permeabilized cells; B – C59 permeabilized cells; C – H295R non-permeabilized cells; D – H295R permeabilized cells. Each point repsent the average of 4 determinations.