Figure 5.

Indirect immunofluorescence staining with the anti-K19M IgG antibody. A, B The H295R and G540 cell lines, paraformaldehyde fixed and non-permeabilized, show a similar granular like surface membrane labeling pattern; C, D, Both in H295R and G540 cultures, paraformadehyde fixed and non-permeabilized, cell footprints are positively labeled; E, F, The H295R and G540 cell lines, formalin/ethanol fixed and permeabilized show a granular cytoplasmic labelling pattern that is perinuclear localized in ER and Golgi network; G, The G59/540 parent line, formalin/ethanol fixed and permeabilized, still shows a small amount of a perinuclear granular staining pattern.