Figure 3.

Marker analysis of chondrocytes derived from MSC. Human bone marrow-derived MSC were differentiated into chondrocytes according to the methods section. A) RT-PCR analyses for chondrogenic markes as is indicated, B) H & E-staining, C) Alcian blue staining (H & E-counterstaining, D) example of negative control for immunohistochemical analyses (DAPI-counterstained), E) immunohiostochemical staining for collagen type II, F) immunohiostochemical staining for chondroitin sulphate 4, G) immunohiostochemical staining for chondroitin sulphate 6, (scale bar = 100 μm).