Table 2

Primers used for RT-PCR of markers for MSC-derived differentiation pathways

Alkaline phosphatase

sense

5'TGGAGCTTCAGAAGCTCAACACCA 3'

368 – 391 NM_000478

antisense

5'ATCTCGTTGTCTGAGTACCAGTCC 3'

798 – 821 NM_000478

Osteocalcin

sense

5'ATGAGAGCCCTCACACTCCTC 3'

19 – 39 NM_199173

antisense

5' GCCGTAGAAGCGCCGATAGGC 3'

292 – 312 NM_199173

Lipoprotein lipase

sense

5'GAGATTTCTCTGTATGGCACC 3'

1261 – 1281 NM_000237

antisense

5'CTGCAAATGAGACACTTTCTC 3'

1516 – 1536 NM_000237

PPARγ2

sense

5'GCTGTTATGGGTGAAACTCTG 3'

128 – 148 NM_015869

antisense

5'ATAAGGTGGAGATGCAGGCTC 3'

458 – 478 NM_015869

Collagen type II

sense

5'GAACATCACCTACCACTGCAAG 3'

4318 – 4339 NM_001844

antisense

5'GCAGAGTCCTAGAGTGACTGAG 3'

4684 – 4705 NM_001844

Collagen type X

sense

5'CCCTTTTTGCTGCTAGTATCC 3'

112 – 132 NM_000493

antisense

5'CTGTTGTCCAGGTTTTCCTGGCAC 3'

556 – 579 NM_000493

Collagen type XI

sense

5'ACTTCTGACTGCCTCTGCTC 3'

5418 – 5437 NM_001854

antisense

5'GCTTTTGCCATGTGATTCTGCC 3'

5891 – 5912 NM_001854

Aggrecan

sense

5'GCCTTGAGCAGTTCACCTTC 3'

1814 – 1833 NM_001135

antisense

5'CTCTTCTACGGGGACAGCAG 3'

2186 – 2205 NM_001135

Chondroadherin

sense

5'ACCTGGACCACAACAAGGTC 3'

535 – 554 NM_001267

antisense

5'CACCTTCTCCAGGTTGGTGT 3'

907 – 923 NM_001267

Fibromodulin

sense

5'CTTACCCCTATGGGGTGGAT 3'

175 – 194 NM_002023

antisense

5'AAGTAGCTATCGGGGACGGT 3'

792 – 811 NM_002023

Schutze et al. Cell Communication and Signaling 2005 3:5   doi:10.1186/1478-811X-3-5