Research
An active form of Vav1 induces migration of mammary epithelial cells by stimulating secretion of an epidermal growth factor receptor ligand
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* Corresponding author: Joan S Brugge jbrugge@hms.harvard.edu
1 Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
2 Current address : Cancer Research, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064, USA
3 Current address : GlaxoSmithKline, Oncology, Collegeville, PA 19426, USA
Cell Communication and Signaling 2006, 4:5 doi:10.1186/1478-811X-4-5
Published: 18 May 2006Additional files
Additional File 1:
Increased migration stimulated by Vav1Y3F expression is blocked by U0126. MCF-10A cells expressing GFP or Vav1Y3F were lifted and pretreated with DMSO (control) or 5 μM U0126 for 30–60 minutes at 37°C. Cells were then seeded in transwells in wells containing assay media plus DMSO -/+ EGF or assay media plus U0126 -/+ EGF as indicated and allowed to migrate overnight. Data are expressed as the percentage of migration of control Vav1Y3F cells in media without EGF (set to 100%) for each individual experiment and are the average plus standard deviation for 3 transwells.
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