An active form of Vav1 induces migration of mammary epithelial cells by stimulating secretion of an epidermal growth factor receptor ligand

doi:10.1186/1478-811X-4-5

Cell Communication and Signaling

Volume 4

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An active form of Vav1 induces migration of mammary epithelial cells by stimulating secretion of an epidermal growth factor receptor ligand

Julie L Wilsbacher¹^,2 , Sheri L Moores¹^,3 and Joan S Brugge¹

¹Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA

²Current address : Cancer Research, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064, USA

³Current address : GlaxoSmithKline, Oncology, Collegeville, PA 19426, USA

author email corresponding author email

Cell Communication and Signaling 2006, 4:5doi:10.1186/1478-811X-4-5


Published:	18 May 2006

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Increased migration stimulated by Vav1Y3F expression is blocked by U0126. MCF-10A cells expressing GFP or Vav1Y3F were lifted and pretreated with DMSO (control) or 5 μM U0126 for 30–60 minutes at 37°C. Cells were then seeded in transwells in wells containing assay media plus DMSO -/+ EGF or assay media plus U0126 -/+ EGF as indicated and allowed to migrate overnight. Data are expressed as the percentage of migration of control Vav1Y3F cells in media without EGF (set to 100%) for each individual experiment and are the average plus standard deviation for 3 transwells.

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