Additional File 1.

Increased migration stimulated by Vav1Y3F expression is blocked by U0126. MCF-10A cells expressing GFP or Vav1Y3F were lifted and pretreated with DMSO (control) or 5 μM U0126 for 30–60 minutes at 37°C. Cells were then seeded in transwells in wells containing assay media plus DMSO -/+ EGF or assay media plus U0126 -/+ EGF as indicated and allowed to migrate overnight. Data are expressed as the percentage of migration of control Vav1Y3F cells in media without EGF (set to 100%) for each individual experiment and are the average plus standard deviation for 3 transwells.

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Wilsbacher et al. Cell Communication and Signaling 2006 4:5   doi:10.1186/1478-811X-4-5