Table 1 |
||||
|
Proliferation, IL-2 and IFN-γ production of stimulated and siRNA-transfected BALB/c T cells. |
||||
|
siRNA |
IL-2 [pg/ml] |
IFN-γ [pg/ml] |
Proliferation [CPM] |
|
|
|
||||
|
αCD3 |
Ctrl |
1417 ± 144 |
965 ± 105 |
4106 ± 433 |
|
Lck |
443 ± 93 |
357 ± 50 |
540 ± 220 |
|
|
PLC-γ |
195 ± 28 |
146 ± 26 |
236 ± 19 |
|
|
ZAP70 |
400 ± 60 |
467 ± 149 |
568 ± 69 |
|
|
|
||||
|
PMA + Ionomycin |
Ctrl |
1980 ± 2 |
5067 ± 346 |
19919 ± 787 |
|
Lck |
2167 ± 0 |
4158 ± 135 |
16640 ± 954 |
|
|
PLC-γ |
1676 ± 28 |
2939 ± 88 |
11285 ± 658 |
|
|
ZAP70 |
1991 ± 2 |
4603 ± 277 |
15987 ± 1906 |
|
|
|
||||
|
T cells were transfected with 0.9 μM Lck-, PLC-γ- and ZAP70-specific siRNA pool, ZAP70-specific stealth™ siRNA 1 and with control siRNA pool (Ctrl). 72 h after nucleofection, functionality of transfected cells was tested by restimulation with anti-mouse CD3 antibody or PMA and ionomycin in vitro. IL-2 and IFN-γ production was determined in the supernatant 24 h after restimulation by ELISA. Means of duplicate values are shown. Proliferation of T cells was detected by 3H-thymidine incorporation 96 h after restimulation. Values of 3H-thymidine incorporation are shown in means of triplicates. |
||||
|
Gust et al. Cell Communication and Signaling 2008 6:3 doi:10.1186/1478-811X-6-3 |
||||
