Figure 4.

Subcellular localization of Nck and the death factor FasL in conjugates of Jurkat T cells and EBV-transformed B-LCL. Upon target cell recognition the death factor FasL is transported to the cytotoxic immunological synapse where it colocalizes with Nck. Jurkat T cells were transiently transfected with FasL, cocultured with superantigen-pulsed B-LCL (*, B lymphoblastoid cell line)), fixed, permeabilized and stained for FasL with anti-FasL mAb NOK-1 and respective AlexaFluor488-conjugated secondary antibodies and for Nck with an anti-Nck pAb and corresponding AlexaFluor546-conjugated secondary reagents.