Table 2

Most important monomeric GFP variants and their spectral properties

Protein

λex

[nm]

λem

[nm]

Relative brightness

[% of EGFP]

Relative photostability

[t1/2 in s] [66]

Reference/source


Blue fluorescent proteins

T-Sapphire

399

511

79

25

[53]


Cyan fluorescent proteins

mCFP

433

475

39

64

[54]

Cerulean

433

475

79

36

[55]

CyPet

435

477

53

59

[56]


Green fluorescent proteins

EGFP

484

507

100

174

BD Biosciences Clontech

Emerald

487

509

116

0.69

[48], Invitrogen


Yellow fluorescent proteins

EYFP

514

527

151

60

[48], Invitrogen

Venus

515

528

156

15

[57]

mCitrine

516

529

174

49

[58]

YPet

517

530

238

49

[56]


Orange and red fluorescent proteins

mKO

548

559

92

122

[59], MBL Intl.

mOrange

548

562

146

9

[60]

tdTomato

554

581

283

98

[60]

DsRed-monomer

556

586

10

16

BD Biosciences Clontech

mStrawberry

574

596

78

15

[60]

mCherry

587

610

47

96

[60]

mPlum

590

649

12

53

[61]


Böhme and Beck-Sickinger Cell Communication and Signaling 2009 7:16   doi:10.1186/1478-811X-7-16

Open Data