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Resolution: standard / high Figure 2.
Immunophenotype of cells obtained from human umbilical cord tissue. A. Flow cytometric analysis of surface antigen expression in the UC-derived cultures
was performed using the labelled antibody anti CD44-PE; anti CD73-PE; anti CD90-FITC;
anti CD105-R-PE. At least 10,000 events are displayed. B. Exemplary CD90 and CD73 expression normalized on cell size: comparison of subpopulations
of small- (filled histogram) and large-sized cells (unfilled histogram). The fluorescence
of the conjugated monoclonal antibodies (anti CD90-FITC and anti CD73-PE) as well
as the forward scatter (FSC) signals were measured on linear scale. The ratios were
calculated by the EPICS XL/MCL flow cytometer (Beckman Coulter).
Majore et al. Cell Communication and Signaling 2009 7:6 doi:10.1186/1478-811X-7-6 |
