Figure 5.

Activation of ΔRaf-1:ER blocks differentiation along the astrocytic lineage in human neural stem cells. (A) HNSC.100 neural stem cells expressing ΔRaf-1:ER were incubated with or without EGF, bFGF and 4OHT, as indicated. RNA was isolated and analyzed by RT-PCR. (B) Quantification and statistical analysis of the data shown in (A) (GFAP, glial fibrillary acidic protein; GAPDH, glycerinaldehyde-3-phosphate-Dehydrogenase; IOD = Integrated optical density) (reproduced from [43], copyright by the American Society for Biochemistry and Molecular Biology).