Skip to main content

PDZ domains and their binding partners: structure, specificity, and modification

Abstract

PDZ domains are abundant protein interaction modules that often recognize short amino acid motifs at the C-termini of target proteins. They regulate multiple biological processes such as transport, ion channel signaling, and other signal transduction systems. This review discusses the structural characterization of PDZ domains and the use of recently emerging technologies such as proteomic arrays and peptide libraries to study the binding properties of PDZ-mediated interactions. Regulatory mechanisms responsible for PDZ-mediated interactions, such as phosphorylation in the PDZ ligands or PDZ domains, are also discussed. A better understanding of PDZ protein-protein interaction networks and regulatory mechanisms will improve our knowledge of many cellular and biological processes.

Introduction

Diverse biological activities are regulated through the dynamic interactions of modular protein domains (e.g., WW, SH3, SH2, PH, and PDZ) and their corresponding binding partners [1]. Elucidation of the specificity, selectivity, and regulatory mechanisms involved in these protein-protein interactions can therefore provide important insights into biological processes such as cell proliferation and cell polarity [1, 2].

PDZ domains are abundant protein-protein interaction modules found in various species (Figure 1) [3–6]. In the mouse genome, for example, 928 PDZ domains have been recognized in 328 proteins, which exist in single or multiple copies or in combination with other interaction modules (Figure 1) [7]. From the abundance and diversity of PDZ domains in cells it is apparent that many cellular and biological functions, especially those involving signal transduction complexes, are affected by PDZ-mediated interactions [7–20].

Figure 1
figure 1

Examples of PDZ domain-containing proteins. Proteins are indicated by black lines scaled to the length of the primary sequence of the protein (from SMART)[184].

PDZ domains are small and often modular entities consisting of 5 or 6 β-stranded and 2 or 3 α-helical structures [21]. PDZ domains typically recognize the extreme C-termini of target proteins [22], but some also recognize the internal sequence motif of target proteins through a single binding site on the domains [23–25]. Structural analysis of PDZ domains and PDZ-mediated interactions by NMR and X-ray crystallographic methods in conjunction with computational methods has provided insights into the specificity or promiscuity of PDZ protein-protein interactions [26, 27]. Proteomic methods, such as large scale protein arrays [28–30] and peptide libraries [31–44], have also been used to understand the binding properties of PDZ protein-protein interactions at a genome-wide level, which may provide clues about novel functions of proteins of interest in various cells. PDZ-containing proteins interact with many proteins within cells, so studying the regulatory mechanisms of PDZ protein-protein interactions, such as phosphorylation, autoinhibition, and allostery, is also vital to understand their biology. This review focuses on the advances made in the fields of structural biology, proteomic applications, and regulatory mechanisms of PDZ-mediated interactions.

Structural characteristics of PDZ domains

At present, more than 200 structures of PDZ domains - either the PDZ domains alone, their complexes with binding partners, or PDZ-PDZ dimers - have been determined by NMR and X-ray crystallography [26]. Small-angle X-ray scattering (SAXS) in combination with NMR has also been used to determine the structure of PDZ-containing proteins [45]. These structural studies provide detailed information on ligand recognition and selectivity of PDZ-containing proteins at the molecular level. In this section, we discuss the recent advances in understanding the structural characteristics of isolated PDZ domains, and PDZ domains in complexes with their binding partners.

PDZ domains structures

Canonical PDZ domains

PDZ domains are usually 80-100 amino acid residues long and adopt a similar topology: structural studies have revealed that canonical PDZ domains are usually composed of 6 β-strands (βA ~ βF), a short α-helix (αA) and a long α-helix (αB) (Figure 2A) [21, 46, 47]. The canonical PDZ family has a highly conserved fold, but secondary structures vary in length [5, 20, 47–49]. The N- and C-termini of canonical PDZ domains are in proximity to each other on the opposite side from the peptide-binding site in a groove between the αB-helix and βB-strand structures (Figure 2A).

Figure 2
figure 2

Structures of PDZ, PDZ-like, PDZ-PDZ dimer, and tandem PDZ domains. (A) Ribbon diagram of Dvl-1 PDZ (PDB code: 2KAW). (B) HtrA2 PDZ (PDB code: 1LCY). (C) ZO-1 PDZ2 (PDB code:2RCZ). (D) GRIP-1 PDZ1+2 (PDB code: 2QT5). The binding site of each PDZ domain is shown by a yellow oval. The structures were generated using the Pymol software.

PDZ-like domains

Similar to canonical PDZ domains, the HtrA family, including HtrA (or DegP), DegS, and DegQ, adopt a PDZ-like fold consisting of 5 β-strands (β1-β5) capped by 2 α-helices (α2 and α3) and also 2 short β-strands at the N and C termini (βN and βC). The well-defined α-helix (α1) is formed in the region between the β1 and β2 loop of the PDZ-like domain (Figure 2B) [17, 41].

Dimerization of PDZ domain

Although most PDZ domains that have been studied in isolation are found to be monomers [5, 20, 47–49], some form dimers [50–55]. Shank-1 PDZ and GRIP-1 PDZ6, for example, form a homodimer via the conserved βB/βC loop and N-terminal βA strands, which display an antiparallel orientation between the βA strands of the proteins [50, 51]. The formation of the PDZ dimer does not affect the binding to its partner because the peptide-binding sites of both PDZ domains remain open. However, the role of both PDZ dimerizations found in vitro remains unclear, because there is no evidence that the full length Shank1 and GRIP1 proteins form functional dimers in vivo.

A novel dimerization mode of PDZ domains has recently been reported in NMR and X-ray crystallographic studies [52–55]. Two independent groups have shown that the second PDZ domain (PDZ2) of the ZO protein forms a dimer by extensive symmetrical domain swapping of β-strands (Figure 2C) [52–55]. The ZO-1-PDZ2 dimer largely maintains the canonical PDZ fold; however, the N- and C-termini of ZO-1-PDZ2 are not close to each other [52–55]. In the ZO-1-PDZ2 dimer, the binding site opens to allow interaction with the target protein [52–54]. In vivo, the presence of the ZO-1 dimer or higher order oligomers was confirmed by the co-immunoprecipitation of fluorescent-labeled ZO-1 (GFP-ZO-1) and endogenous ZO-1 [52].

Tandem PDZ domains

Although isolated PDZ domains usually fold into a well-defined native structure, recent studies have shown that some PDZ domains need other PDZ domains that are connected to them in a tandem arrangement in order to fold properly [56, 57]. For example, the first and second PDZ domains (PDZ12) of GRIP-1 proteins are connected in this way (Figure 1). Structural studies have revealed that the isolated PDZ1 of GRIP-1 is not well folded, but that the PDZ12 tandem is (Figure 2D), indicating that the folding of PDZ1 strictly depends on the covalent attachment of PDZ2 [56]. Another example of a tandem PDZ domain is the fourth and fifth PDZ domains (PDZ45 tandem) of GRIP-1, which are required to interact with GluR2/3 [57]. The solution structure of the PDZ45 tandem shows that PDZ4 contains a deformed binding groove, but that this deformed groove stabilizes the structure of PDZ5.

Besides these examples, recent studies reported the structures of tandem PDZ domains present in PSD-95 [58], human synthenin [59, 60], and X11a [61], showing that tandem PDZ domains play crucial roles in the formation of structural and functional supramodules [56–63].

The carboxylate-binding site of PDZ domains

PDZ domains have a single binding site in a groove between the αB and βB structural elements with a highly conserved carboxylate-binding loop (R/K-XXX-G-Φ-G-Φ motif, where X is any amino acid residue and Φ is hydrophobic residues) located before the βB strand [4, 31]. The first Gly residue in this motif is variable among canonical PDZ domains, and can be replaced by a Ser, Thr, or Phe residue (Figure 3A) [26]. The second and the fourth residues are hydrophobic, such as Val, Ile, Leu, or Phe (Figure 3A). The side chains of both of these residues create the hydrophobic binding pocket of canonical PDZ domains [5].

Figure 3
figure 3

The amino acid residues in the carboxylate-binding loop regions of PDZ domains adopt a specific conformation. (A) Selected 'X-Φ-G-Φ' motifs from known structures of PDZ domains, where X represents any amino acid residue and Φ represents hydrophobic amino acid residues. (B) 4 Top boxes: Distribution of the (ϕ,ψ) angle of the amino acid residue at the specific position. Bottom: Ribbon diagram of the Dvl1 PDZ domain (grey, PDB code: 2KAW); the X1-Φ2-G3-Φ4 motif is highlighted (stick).

Because this loop region of PDZ domain plays a key role in ligand binding, the conformational properties of amino acid residues in this region of multiple currently deposited PDZ domain structures were analyzed. Interestingly, the second residue in the loop region adopts the α-helix conformation whereas the fourth residue adopts the β-sheet conformation (Figure 3B). The carboxyl oxygen atom of the second residue in the loop region forms a hydrogen bond with a residue in the αA helix, thereby stabilizing this short helix. The third Gly residue in the loop region is fully conserved (Figure 3A) and adopts the left-handed α-helical conformation in structures available to date (Figure 3B), which may be important for determining the PDZ fold. These specific conformations for each residue in the carboxylate-binding loop region allow amide groups to serve as the H-bonding donors (Figure 3B).

As seen in canonical PDZ domains, PDZ-like domains also have a single binding site located in the groove between the β1 strand and the α3 helix structures. The carboxylate-binding loop region [X1-Φ2-G3-Φ4 motif] in PDZ-like domains is located before the β1 strand (Figure 2B), and the third Gly residue is highly conserved in this loop region. The second and fourth residues in the loop regions are hydrophobic residues, as seen in canonical PDZ domains.

Conformational properties of peptides bound to PDZ domains

C-terminal regions of proteins interacting with PDZ domains

To date, approximately 20 structures of PDZ domains in complex with their binding peptides, mostly complexes with free COOH-terminal peptides, have been reported [26]. The binding peptide forms an additional β strand in the groove between the βB strand and the αB-helix structure of the PDZ domain [4]. As the conformational properties of the bound PDZ ligands might be useful to understand, the binding specificity of PDZ domains, the backbone (ϕ,ψ) dihedral angles for each position of the amino acid residues were investigated (Figure 4A). Bound peptides that form complex structures with PDZ domains have 4 or 9 residues. The (ϕ,ψ) angles at the p(0) site of the PDZ ligand (i.e. the most C-terminal residue) are randomly scattered in the Ramachandran plot and do not show preference for a specific conformation. The (ϕ,ψ) angles at p(-1), p(-2), and p(-3) sites are located on the β strand (βS) or extended (ε) conformation regions [64]. Interestingly, for the complex structure of the PDZ domain of Dishevelled with the Dapper peptide, the p(-3) residue adopts the right α-helix (αR) conformation, which contrasts what is seen in other PDZ-mediated interactions (see also Figure 4B) [65]. Positions -4 and -5 of amino acid residues adopt the β-strand or extended conformation, but some positions have different conformations. Taken together, the difference in conformational properties of each residue at the different positions may explain the binding specificity of PDZ domains (Figure 4A).

Figure 4
figure 4

Each residue in the PDZ-bound ligand adopts a specific conformation. (A) Distribution of (ϕ,ψ) angles of each residue of the PDZ-bound ligand. Dataset were from PDBsum http://www.ebi.ac.uk/pdbsum/. (B) The orientation of each residue in the side chain of the PDZ ligand in case of a free carboxy-terminal peptide and the internal sequence from PSD-95/nNOS and Par-6/Pals1 complex [65, 69, 185, 186].

Internal sequence of target proteins

Although the binding of many PDZ-containing proteins occurs by recognition of the extreme C-terminus of target proteins [31], some PDZ domains can also bind to internal sequences of target proteins [48, 49, 66–70]. The most well-characterized example of this nature is the interaction between the PDZ domain of the syntrophin (or PSD-95) protein with the internal β-hairpin finger structure of the nNOS protein (Figure 4B) [66–68]. This nNOS β-hairpin pockets in the binding site of syntrophin (or PSD-95) protein, which mimics a normal peptide ligand, but the extreme C-terminus is replaced with a sharp β-turn [68]. Another example is the interaction of the internal ligand of the Pals1 protein with the Par-6 PDZ domain (Figure 4B) [69]. Prehoda and coworkers determined the three-dimensional structure of the Par-6 PDZ domain in complex with the Sdt/Pals1-derived peptide Ac-Y-8P-7K-6H-5R-4E-3M-2A-1V0D+1C+2P+3CONH2 by X-ray crystallography. The Pals1 internal ligand adopts the extended conformation, which is comparable to that seen in the nNOS-PSD-95 internal interaction [66, 69]. The binding of the Pals1 internal ligand induces a conformational change in the carboxylate-binding loop of the PDZ domain of Par-6, which may result from the formation of salt bridges between the Asp(+1) residue from the internal ligand and a Lys residue from the carboxylate-binding loop, as indicated by alanine scanning mutagenesis experiments [69]. Besides these 2 interactions with internal peptides, several others have also been reported: binding of the PDZ of Dvl with the internal KTxxx(W/I) motif of Frizzled and Idax proteins [48, 70], the PDZ binding of nNOS to the internal -[D/E]-x-F-[D/E]- motif of Vac14, and the PDZ interaction of HtrA1/2/3 with internal sequences of misfolded polypeptides [41]. Whether the internal sequences of target proteins adopt a specific conformation in the bound state remains to be determined.

Interactions among residues in the PDZ - peptide complex

As the C-terminal region of PDZ-binding proteins forms an additional β-strand in the groove between the βB-strand and the αB-helix structure of the PDZ domain [4], each residue in the PDZ ligand can interact with specific residues in the binding pocket of the PDZ domain (Figure 4B). This section summarizes the structural characteristics of these specific interactions among the side chains of PDZ ligands and the binding surfaces of PDZ domains (Figure 4B).

Structural analyses have shown that the p(0) side chain of the PDZ ligand interacts with βB-1, αB-8, and αB-5 side chains of the PDZ domain [32, 36, 37, 41, 42, 71–73]. The numbers used here in combination with the structural elements represent the position of the relevant amino acid residue on a specific secondary structure element: for example, βB-1 is the first residue of the βB structure. The preference of the p(0) residue is likely related to the size of the βB-1 side chain [36]. If βB-1 is a Phe residue, the p(0) site of the PDZ ligand prefers a Val residue over a bulky residue; on the other hand, if βB-1 is a Leu/Ile residue, the p(0) site of the PDZ ligand prefers bulky residues [73].

The p(-1) side chain of the PDZ ligand may interact with the βB-2 and βC-5 residues or a residue of the βC-αA loop regions, or both, within the PDZ domain. As the p(-1) residue of the PDZ ligand is exposed to the solvent, the residue was initially thought to have no preference. Accumulating evidence, however, shows that some PDZ domains favor specific residues at the p(-1) position [36, 41, 42, 73–75]. For example, the Erbin and Dishevelled PDZ domains prefer a Trp residue at p(-1) [36, 46]. To understand why the Trp(-1) residue is preferred in the binding of Dvl-1 PDZ to the VWV tripeptide, its complex structure was determined by NMR spectroscopy, followed by molecular dynamic simulation and assessment of the molecular mechanics with the Poisson-Boltzmann surface area method [46]. The results showed that hydrophobic interactions contribute to the increased binding affinity of the Dvl PDZ/the VWV tripeptide [46]. For the preferred Trp of the p(-1) site for the Erbin PDZ ligand, Beuming et al. (2009) predicted a favorable release of high-energy water molecules into bulk [76]. Despite the preference for the W(-1) residue in some PDZ ligands, PDZ domains with Cys residue at βB-2 position likely favor the Cys residue at the p(-1) site in the PDZ ligand [77]. For example, the N-terminal PDZ domain of InaD forms the complex with the C-terminus of NorpA through disulfide bond formation between the Cys residue at p(-1) site in PDZ-ligand and the Cys residue at βB-2 position of the PDZ domain [77]. Kimple et al. (2001) proposed that some PDZ domains may also form an intermolecular disulfide bond between a PDZ domain and its binding ligand [77].

The p(-2) residue in the PDZ ligand can interact with αB-1 and αB-5 residues on the PDZ domain, which plays an important to role in determining the binding specificity of PDZ-mediated interactions [4, 31, 73]. The preference for the p(-2) residue is likely related to the physicochemical properties of αB-1 and αB-5 residues. It has been suggested, for example, that the preference for the Ser or Thr residue at the p(-2) site in the PDZ ligand is due to hydrogen bond formation with the side chain of the His residue at αB-1 [78]. The hydrophobic properties of αB-5 residue may explain the preference of the Thr residue over the Ser residue at the p(-2) site in the PDZ ligand [39, 79].

For the p(-3) residue in PDZ ligands, it appears to be difficult to define strict parameters for the interaction. It can interact with the βB-4 for short ligand side chains or the βB-5 residue for long ligand side chains [36, 41, 80]. However, the p(-3) residue of the PDZ ligand, dapper, is in proximity to the αB-1 residue (Asn) on the Dvl PDZ domain (Figure 4B) [65].

Characterization of PDZ-mediated interactions with advanced tools

While the complex structures of PDZ domains and their ligands by NMR and X-ray provide molecular details of PDZ-mediated interactions, advanced tools such as proteomics and protein arrays have been developed to characterize the PDZ-mediated interaction network proteome-wide. This section summarizes techniques such as yeast two-hybrid (Y2H), coimmunoprecipitation, protein microarray, and peptide libraries and their applications in studying the PDZ-mediated interactions [79, 81–87]. We summarize the classification of PDZ domains investigated by peptide library approaches and suggest a need to deposit the accumulated information obtained by these advanced tools into publicly available databases to accelerate the identification of novel PDZ-mediated interactions.

Techniques for studying the PDZ-mediated interactions

Y2H approach

The Y2H approach is widely used to identify protein-protein interactions [79, 81–86]. In a study of PDZ-mediated binding events by Lee and coworkers, the C-terminal fragment of target proteins was subcloned into a bait vector containing a DNA-binding domain, and the PDZ domains subcloned into the matching prey vector containing the corresponding activation domain [84]. Both partial fusion proteins were expressed in the same yeast cell and their binding reconstituted a functional transcription activator, which led to transcriptional activation of a reporter gene. Gisler et al. (2008) developed a modified membrane yeast two-hybrid (MYTH) system to test interactions between full-length integral membrane proteins and their cognate PDZ-interacting partners [85]. However, Y2H approaches have a high rate of false positives and false negatives, and therefore their results need to be interpreted with caution [82, 83].

Co-immunoprecipitation (co-IP) approach

In co-IP, it is attempted to identify a specific protein as interaction partner of another in cellular or tissue protein extracts [84]. The protein complex is immobilized via a specific antibody on protein A or protein G Sepharose beads and unbound proteins are removed by a series of washes. The protein complex is then eluted from the beads and analyzed by SDS-PAGE followed by Western blotting with specific antibodies for the bait and prey [84, 88]. Co-IP is usually used as a complementary assay to confirm the direct interaction identified by other biophysical methods [81, 84, 86, 89–92]. Gee et al. (2009), for example, identified a direct interaction of the C-terminus of the vasoactive intestinal polypeptide (VIP) type -1 receptor (VPAC1) and the PDZ domain of the synaptic scaffolding molecule (S-SCAM) by an Y2H screen, which was then confirmed by co-IP in HEK293 mammalian cells and human pancreatic and colonic tissues [92].

PDZ domain arrays

PDZ domain arrays have been developed by several groups [28–30]. Hall and coworkers developed a proteomic array of 96 putative class I PDZ domains derived from cytoplasmic proteins [28]. These PDZ domains were expressed as His- and S-tagged fusion proteins [93], purified, and spotted onto 96-grid nylon membranes [28]. Tymianski and coworkers cloned all publicly known human PDZ domains (more than 160 constructs) into expression vectors and produced GST-PDZ fusion proteins, which were arrayed into 96-well plates [94].

Because approximately 20% of GPCR proteins (including ionic channels, ionotropic receptors, and single transmembrane proteins) in the human genome have a PDZ-binding motif, PDZ domain arrays have been repeatedly used to identify the binding partners of GPCR proteins [28–30]. For example, Hall and coworkers demonstrated in this way that the C-termini of GPCR proteins such as P2Y1R, mGluR5, and β1AR are binding partners for several PDZ domains [28–30]. These PDZ protein-protein interactions were subsequently confirmed by coimmunoprecipitation and immunofluorescence co-localization studies [28–30].

PDZ domain arrays also have diagnostic applications and can be used to develop biomarkers for detecting viral infections. For example, in a large-scale sequencing analysis of avian influenza viruses (AIVs), Naeve and coworkers found that the multifunctional NS1 protein has a PDZ-binding motif at the last C-terminal sequence [95]. By using commercial PDZ domain arrays containing 123 PDZ domains derived from the human genome, they showed that the full-length avian NS1 protein binds to 30 different human PDZ domains, whereas the human NS1 protein binds very weakly or not at all [95]. These results indicate that avian NS1 proteins, not human NS1 proteins, bind to and inhibit many PDZ interactions. Lamb and coworkers verified the importance of these PDZ-mediated interactions in a mouse model [96]. A company has recently developed a rapid antigen assay test to detect the avian influenza virus in humans using its own PDZ array http://www.cdc.gov/EID/content/14/3/ICEID2008.pdf.

Although most PDZ domain arrays developed to date provide qualitative information on PDZ protein-protein interactions, PDZ domain arrays and fluorescence polarization can be combined to generate quantitative information of PDZ-mediated interactions. This information can be used to further elucidate the specific binding properties of PDZ domains [97]. For example, MacBeath and coworkers generated microarrays containing 157 mouse PDZ domains [97]. These PDZ domains were arrayed onto aldehyde-presenting flat glass slides, and multiple identical microarrays were printed in a 96-well plate-like format [97, 98]. They also synthesized and purified 217 fluorescently labeled peptides derived from the C-terminal residues of mouse proteins. All possible interactions of these 157 PDZ domains with the 217 genome-encoded peptides were then examined by the fluorescence polarization assay [97]. The PDZ domains microarrays identified interactions of moderate to high affinity (KD ≤ 10 μM) in a high-throughput format, with a moderate false-positive rate of 19% and an even lower false-negative rate of 14% [98]. The results were subsequently used to build a model with a position-specific scoring matrix (PSSM) that predicts the selectivity of the PDZ domain [97, 99]. Using this model, MacBeath and coworkers screened 31,302 peptide sequences corresponding to the C-termini of all translated open reading frames in the mouse genome and found no less than 18,149 PDZ-peptide interactions. This suggests that obtaining comprehensive information on PDZ-peptide interactions may be very helpful in supporting future biological investigations of target protein functions [97, 99].

Peptide library approaches: Phage display and SPOT synthesis

Because PDZ domains recognize only short linear motifs in their target proteins, peptide library approaches are being used to define the binding specificity of PDZ domains, to confirm known PDZ interactions, to optimize the PDZ-binding ligands, and to find putative PDZ-binding partners [31–44, 100].

Phage display is a high-throughput approach in which libraries of more than 1011 random peptides or proteins are expressed on the surfaces of phage particles, which harbor short randomised DNA stretches that encode for the oligopeptide to be displayed for studying PDZ-ligand interactions [32]. After typically several rounds of 'panning' the binding peptide candidates are identified by isolating single phages and sequencing their DNA [101]. Given that most PDZ domains recognize the free C-termini tail of target proteins, C-terminally displayed peptides have been developed [31, 32, 39, 40, 73, 102]. Songyang et al. (1997) examined peptide-binding specificities of 9 PDZ domains by using the oriented peptide library to elucidate relative preferences for certain amino acids at a given position of PDZ-binding ligands [31]. Kurakin et al. (2002) developed the target-assisted iterative screening (TAIS) method, a simple and rapid 2-step procedure for in vitro affinity selection of specific binding partners from molecules with enormous molecular diversities to the target molecule of interest [34]. This method has been applied to a commercial phage-displayed cDNA library with a PDZ domain as a target to investigate the selectivity and promiscuity of the interactions [39]. Tonikian et al. (2008) used C-terminal phage-displayed random peptide libraries containing greater than 10 billion random peptides to analyze the binding specificities of 145 PDZ domains (from 57 C. elegans and 88 human proteins) [73].

SPOT synthesis allows the parallel synthesis and screening of thousands of cellulose membrane-bound peptides, and has been applied to study PDZ-mediated interactions [44, 103]. For example, Wiedemann et al. (2004) generated a peptide library comprising 6223 C-termini of human proteins by SPOT synthesis of inverted peptides to obtain an overview of the space of target sequences for 3 PDZ domains from AF6, ERBIN, and SNA1 proteins, respectively [103]. On the basis of the ligand preferences detected for these PDZ domains, they designed focused peptide libraries (profile libraries) and quantified the binding affinity contributions of the 4 C-terminal ligand residues. The authors studied the binding specificities of PDZ domains and established the relationship between the C-terminal ligand sequences and the corresponding KD values. Finally, they predicted putative PDZ-binding partners on the basis of the SWISS-PROT database.

Classification of PDZ domains

Recent studies on proteomic applications that use protein arrays and peptide libraries have generated a wealth of information on protein-protein (or peptide) interactions (PPIs) [71, 72, 93–95]. In addition, the existing classification of PDZ domains - (1) class I domains, which recognize the motif S/T-X-Φ; (2) class II domains, which recognize the motif Φ-X-Φ; and (3) class III domains, which recognize the motif D/E-X-Φ as their preferred C-terminal motif, where Φ represents a hydrophobic residue - has been challenged because of the importance of other upstream positions within the PDZ ligand, such as -3 or -4 position (position 0 referring to the C-terminal residue), to the binding specificity of target proteins [36, 42, 71, 73, 74, 104]. By analyzing a total of 72 PDZ domains corresponding to 2,998 ligands, Tonikian et al. (2008) suggested the 16 classes of PDZ domains, which are defined by the following C-terminal motifs: 1a (ϕ-[K/R]-X-S-D-V); 1b (Ω-[R/K]-E-T-[S/T/R/K]-ϕ); 1c (ϕ-ϕ-E-T-X-L); 1d (E-T-X-V); 1e (T-W-ψ); 1f (Ω-Ω-T-W-ψ); 1g (ϕ-ϕ-ϕ-[T/S]-[T/S]-Ω-ψ]; 2a (F-D-Ω-Ω-C); 2b(W-X-Ω-D-ψ); 2c (W-Ω-ϕ-D-ψ); 2d (ϕ-ϕ-X-[E/D]-ϕ-ϕ-ϕ); 2e (ϕ-ϕ-ϕ-ϕ); 2f ([D/E]-ϕ-Ω-ϕ); 3a (Wx[S/T]-D-W-ψ); 4a (Ω-ϕ-G-W-F); ϕ, hydrophobic (V, I, L, F, W, Y, M); Ω, aromatic (F, W, Y); ψ, aliphatic (V, I, L, and M); and X, nonspecific [73]. They also suggested that their specific map for the PDZ domain family will be able to predict natural protein interactions [73]. Further studies with independent methods would be necessary to verify their classification, because the 72 PDZ domains investigated by Tonikian et al. (2008) may not be enough to represent the whole PDZ 'domainome'.

Bioinformatics and other methods for finding putative PDZ-binding partners

Studies with PDZ microarrays and peptide libraries have focused on generating information on PDZ-mediated interactions, generating a key resource to investigate biological networks and signaling pathways within cells [28–44, 97–99]. This information needs to be comprehensively deposited in publicly available repositories, such as iSPOT, DOMINO, and PDZBase [105–108], in order to maximally accelerate the discovery of novel PDZ-mediated interactions in cells. PDZBase is a unique database that contains information extracted from the literature of all known PDZ domain-mediated protein-protein interactions obtained from in vivo (coimmunoprecipitation) or in vitro experiments (GST-fusion or related pull-down experiments) [108].

However, the information on interactions derived from high-throughput methods should be interpreted with caution and verified by other independent methods, such as Y2H and co-IP. For example, MacBeath and coworkers predicted 18,149 PDZ-peptide interactions from PDZ microarrays [97, 98]. Among them, 710 proteins were proposed to be binding partners for the Dvl PDZ domain [97]. Because Dvl proteins (Dvl1, Dvl2, and Dvl3 in mammals) play diverse roles in Wnt signaling [109], identification of their binding partners is key to understanding their biological functions. Although we cannot exclude the possibility that unexpected Dvl-binding proteins may exist in the predicted data [97], no known Dvl PDZ-binding partners such as Dapper [65] and Daple [110] have been found in this data set, highlighting the importance of further verification of the proposed binding partners by other methods. Furthermore, there is a discrepancy between the list of predicted Dvl PDZ binding partners reported by Tonikian et al. (2008) who were using phage-displayed oriented peptide libraries and that by Stiffler et al. (2007) resulting from the use of PDZ domain microarrays [97]. Ten potential binding partners of the Dvl2 PDZ domain predicted by Tonikian et al. are not found in the prediction list of Stiffler et al. In addition, because neither study considered the expression profiles and subcellular localizations of the proposed PDZ-binding partners, the number of real binding candidates for a specific protein is expected to be significantly lower than that reported. siRNA experiments are needed to verify putative interactions in vivo. Along these lines, Cui et al. (2007) conducted a proteomic analysis of the interactions of neuronal signaling proteins with human PDZ domains (>6,500 interactions) using an ELISA-based assay [94]. They found that the Tat-NR2B9c peptide, which is a Tat peptide consisting of the nine COOH terminal residues of the NR2B subunit, binds specifically to PSD-95 family members (PSD-95, PSD-93, SAP97 and SAP102) and Tax interaction protein 1 (TIP1). As they suppressed the Tat-NR2B9c-binding proteins in primary murine neuron culture by RNA interference, remarkably, neurons lacking PSD-95 or nNOS, but no other PDZ domains, exhibited reduced excitotoxic vulnerability [94].

Taken together, optimal use of all the databases compiling the interactions obtained by different methods will reduce the time and expense of finding a specific PDZ-binding partner for further studies at a genome-wide level, and will also aid its functional characterization [24, 73, 111].

Regulation of PDZ-mediated interactions

As PDZ domains interact with many proteins, understanding the regulatory mechanisms of PDZ-mediated interactions is important to gain insight into biological processes. Posttranslational modification, autoinhibition, and allosteric interaction have been proposed to regulate PDZ-mediated interactions.

Phosphorylation within the PDZ ligand modulates PDZ protein-protein interactions

Phosphorylation of Ser, Thr, or Tyr within the PDZ ligand can modulate PDZ-mediated interactions (Table 1 and Figure 5). For example, the interaction between the NR2B subunit of the NMDA receptor with PSD-95 is negatively modulated by phosphorylation (Figure 5A). The PDZ ligand (LSSIES DVCOOH) of NR2B at the p(-2) site is phosphorylated by CK2 in vivo (although S(-2) does not match the substrate consensus motif of CK2), which disrupts its interaction with PSD-95 and decreases the surface expression of NR2B in neurons [112]. The authors also reported that CK2 colocalizes with NMDAR in dendrites and at some excitatory synapses [112, 113]. Kim and coworkers showed that phosphorylation by PKA at the p(-2) site within the PDZ ligand (ANRRTT PVCOOH) of stargazin, which is a transmembrane AMPA receptor regulatory protein, abrogates its binding to PSD-95 PDZ1 domains and thereby regulates synaptic AMPAR function [114, 115]. The disruption of PDZ-mediated interactions by phosphorylation can be rationalized by the elimination of a possible hydrogen bond donor sidechain; the side chain of an unmodified Ser or Thr residue at the p(-2) can form a hydrogen bond with the N-3 nitrogen of the His residue at position αB-1 for the PDZ class I domain, and phosphorylation of the PDZ ligand in this position destroys this possibility, resulting a in loss of PDZ-based interactions [78].

Table 1 Phosphorylation of the PDZ ligand by serine or threonine kinase modulates PDZ protein-protein interactions
Figure 5
figure 5

Posttranslational modifications on the PDZ ligands or PDZ domains modulate PDZ protein-protein interactions. (A) Phosphorylation of the PDZ ligand or PDZ domain inhibits PDZ interactions. The cross represents a reduced or abolished interaction. (B) Formation of intramolecular disulfide bond (symbol, 'ox') in the PDZ domain prevents binding of the other binding partner. (C, D) Phosphorylation or competitive binding changes the autoinhibited conformation.

Besides phosphorylation of the p(-2) site within the PDZ ligand, other positions in the ligand can also be phosphorylated [55, 86, 116, 117]. The Ser residue at p(-4) site in the C-terminal PDZ ligand (YNYGIES VKICOOH) of the AMPA receptor GluR2 subunit is phosphorylated by PKC in vitro and in vivo [116, 117]. Coimmunoprecipitation and in vivo binding studies have shown that phosphorylation significantly decreases binding of GluR2 to the PDZ domain of GRIP1/2 but not of PICK1. Lin and Huganir reported that phosphorylation of GluR2 and binding to PICK1 dynamically regulate GluR2 recycling [118]. Tian et al. (2006) showed that CaMKII phosphorylates the C-terminal cytoplasmic region of LRP4 at Ser-1900, p(-5) site, of the C-terminal tail (ERKLS SESQVCOOH), which suppresses the interaction of the protein with PSD-95 and SAP97 [119]. The reason for the decrease in PDZ binding affinity by phosphorylation at the -4 and -5 positions of residues in the PDZ ligand remains unclear. Zhang and coworkers have shown by structural and biochemical studies that domain-swapped dimerization of the ZO-1 PDZ2 domain plays a crucial role in the interaction with the C-terminus of the connexin43 protein (referred to as Cx43 peptide, ASSRPRPDDLEI) [55]; this interaction is regulated by phosphorylation of Ser residues at the -9 and -10 positions in the PDZ ligand of Cx43. These Ser residues are substrates for the kinases Akt and PKC [120–125]. NMR studies suggest that the phosphorylation of the Ser residues at p(-9) and p(-10) sites may interfere with the charge-charge interaction network formed by Cx43 and the residues at the dimer interface of ZO-1 PDZ2 [55].

To examine the effect of ligand position-dependent phosphorylation of the PDZ ligand, Volkmer and coworkers developed a modified SPOT synthesis technique that generated 3 arrays, each containing the 100 PDZ-binding sequences and also all possible phosphorylated variants for the 3 PDZ domains from AF-6, ERBIN, and SNA-1 proteins [38]. The interactions of 344 peptides for AF-6 PDZ, 319 peptides for ERBIN PDZ, and 355 peptides for the SNA-1 (α-1-syntrophin) PDZ domains showed that phosphorylation of the PDZ ligand at p(-2) (<50% residual binding activity [rba]) and at p(-1) (~50% rba) significantly inhibited PDZ-mediated interactions; phosphorylation at p(-4), (-7), and (-8) only slightly affected the interactions (~80% rba), depending on the PDZ domain; and phosphorylation at p(-3), (-5), (-6), (-9), or (-10) had little or no influence on the interactions (>80% rba). Although the PDZ domain of AF-6 is recognized as a class II PDZ domain, phosphorylation at p(-2) site disrupts the interaction between AF-6 PDZ and the C-terminal ligand (ST EV) of BCR (~30% rba). Data on the phosphorylation sites of PDZ ligands and the roles of phosphorylations of the PDZ ligands will be useful to elucidate the regulatory mechanism of PDZ-mediated interactions, even if the kinases that phosphorylate the PDZ ligands remain unknown.

While many studies have reported that phosphorylation at the C-terminus of proteins negatively modulates PDZ interactions, others have shown that phosphorylation can also promote PDZ interactions [86, 126]. Interestingly, a study by Roche and coworkers documented that phosphorylation of a PDZ-binding motif did not affect PDZ interactions: phosphorylation by PKA or PKC of the p(-6) site within the C-terminus of the NR2C subunit of NMDAR did not change the binding of the PSD-95 PDZ3 or the surface expression of NR1/NR2C NMDA receptors [127]. Surprisingly, a phosphomimetic mutation accelerated channel kinetics, suggesting that phosphorylation alters the function of NMDA receptor channels. These results suggest that phosphorylation of the PDZ binding motif of a target protein might not affect association with the PDZ domain but can still play a role in the functioning of its target protein.

Disulfide bond formation blocks PDZ protein-protein interactions

Although phosphorylation is important in regulating PDZ protein-protein interactions, intramolecular disulfide bond formation in PDZ domains can also modulate binding [128, 129]. For example, the PDZ5 domain of InaD, a multiple PDZ domain-containing protein in photoreceptor cells of the fruit fly (Figure 1), exists in a redox-dependent equilibrium between 2 conformations: the reduced form, which is similar to the structure of other PDZ domains, and the oxidized form, in which the ligand binding site is distorted through formation of a strong intramolecular disulfide bond between 2 cysteines situated in the βC strand and the αB helix (Figure 5B) [128, 129]. This provides the first evidence that disulfide bond formation is able to change the conformation of the PDZ domain and to regulate its function.

Phosphorylation on the PDZ domain itself negatively modulates PDZ interactions

Several studies have reported that phosphorylation on the PDZ domain itself may also disrupt PDZ protein-protein interactions (Figure 5A and 5C). For example, Luca and coworkers found that activation of the NMDA receptor induces a CaMKII-dependent phosphorylation of SAP-97 or PSD-95 [130]. The protein SAP-97 is directly associated with NR2A protein through its PDZ1 domain, and phosphorylation of Ser-232 in SAP-97 by CaMKII disrupts NR2A interaction both in vitro and in vivo. The authors also identified a CaMKII-dependent phosphorylation on the PDZ domain of PSD-95 [130]. CaMKII phosphorylation of Ser-73 of PSD-95 causes NR2A dissociation from PSD-95, but does not interfere with the binding of NR2B to PSD-95 [130]. This phosphorylation of PSD-95 negatively regulates spine growth and synaptic plasticity [131]. Remarkably, Ser-232 in the PDZ1 domain of SAP-97 and Ser-73 in PDZ1 domain of PSD-95 are located in the αB-helix structure, which is the binding site of the PDZ domain. These results suggest that phosphorylation at a Ser or Thr residue of the binding sites of PDZ domains plays an important role in regulating PDZ-mediated interactions.

Another example is the phosphorylation site (Ser-77) of the first PDZ domain (PDZ1) of NHERF-1, a signaling adaptor protein containing 2 PDZ domains at the N-terminus and an ezrin-radixin-moesin (ERM) domain-binding (EB) region at the C-terminus [132, 133]. The phosphorylation of Ser-77, located on the αB-helix on the PDZ domain, by protein kinase C (PKC) attenuates its binding to physiological targets such as the β2-adrenergic receptor and sodium-phosphate cotransporter type IIa (Figure 5C) [133, 134]. The phosphorylation at Ser-162 of the second PDZ (PDZ2) domain in NHERF-1 has also been reported [135]. Raghuram et al. (2003) showed that this phosphorylation lowers the PDZs affinity for the CFTR C terminus and disrupts the bivalent PDZ domain interaction of NHERF-1 (Figure 5C) [135].

Autoinhibited conformation of PDZ-containing proteins

Some PDZ-containing proteins have a PDZ-binding motif at their C-terminal tail. The binding site of the PDZ domain in these proteins can be occupied by their own C-terminal sequences, thereby inhibiting the binding of the PDZ ligand [45, 61, 136–141]. Here, we discuss three PDZ-containing proteins that adopt the auto-inhibitory conformation.

NHERF-1

Two independent groups have reported that the C-terminal tail of the NHERF-1 protein binds to its own PDZ2 domain (Figure 5C) [45, 136]. Data from solution SAXS show that the C-terminal EB region in NHERF-1 folds back to PDZ2 [136], which is supported by recent NMR and circular dichroism (CD) studies showing the presence of specific intramolecular interactions between PDZ2 and the C-terminal EB regions [139]. Remarkably, the last residues in the C-terminus of NHERF-1 adopt an α-helix conformation when bound to PDZ2, which is comparable to an extended conformation of a typically bound PDZ ligand [138, 139] and this α-helical conformation of the EB region is accommodated in the peptide binding pocket of PDZ2. GST pull-down experiments and surface plasmon resonance (SPR) experiments indicate that the intramolecular interactions between PDZ2 and the EB region compete with the binding of extrinsic ligands [45, 139]. In addition, studies on the effect of phosphorylation on the autoinhibitory conformation of NHERF-1 by solution SAXS and binding assays suggest that this conformation could be disrupted by protein kinase C (PKC) phosphorylation at Ser-339 and Ser-340 in the C-terminal domain of NHERF-1. In line with this, the PKC phosphorylation-mimicking mutant NHERF(S339D/S340D) displays a higher binding affinity for its extrinsic ligand C-CFTR than wild-type NHERF-1 does [136]. The autoinhibited conformation of NHERF-1 can also be regulated by the binding of ezrin (Figure 5C). Li et al. (2009) used small-angle neutron scattering (SANS) to demonstrate that the C-terminal EB region binds to ezrin, which induces conformational changes in two region of NHERF-1: the region linking PDZ2 and the C-terminal EB region and also that linking the PDZ1 and PDZ2 domains [140]. The authors suggest that this long-range interdomain conformation in NHERF1 bound to ezrin increases the binding capabilities of both PDZ domains [140].

The X11α protein, involved in regulating neuronal signaling, trafficking and plasticity[142], contains a central PTB domain and 2 C-terminal PDZ domains (PDZ1 and PDZ2 arranged in tandem). Zhang and coworkers found that the C-terminal tail of X11α folds back and binds to the first PDZ domain, suggesting that the binding site of PDZ1 is closed (Figure 5D) [61]. The authors hypothesize that phosphorylation on the C-terminal tail of X11α might re-open the binding site of the PDZ1 domain. To test this hypothesis, they made a phosphorylation mimic peptide wherein the highly conserved Tyr(-1) residue was substituted with the Glu(-1) residue at the C-terminal tail of X11α. Interestingly, this mutant peptide did not bind to the PDZ1 domain but did bind to the PDZ2 domain of X11α, suggesting that phosphorylation might lead to conformational changes in the autoinhibited PDZ-containing protein and also changes in the binding selectivity of PDZ domains in X11α. However, the tyrosine kinase driving this phosphorylation remains to be determined (Figure 5D).

Tamalin, which is also called the GRP1-associated protein [143], contains an N-terminal alanine-rich region, a central PDZ domain, and a C-terminal Leu-zipper domain (Figure 1) [144–146]. Sugi et al. (2007) have reported the crystal structure of the autoinhibitory PDZ domain of tamalin [141]. In the absence of mGluR protein, tamalin self-assembles into an autoinhibited conformation through its PDZ domain and its C-terminal PDZ ligand. The C-terminus of mGluR protein can competitively bind to the PDZ domain of tamalin at a high concentration, thereby disrupting weak inhibitory interactions, suggesting that the PDZ domain of tamalin switches between the trafficking-inhibited and -active forms, depending on the association with mGluR [141].

Allosteric regulation of PDZ-mediated protein interactions

Recent studies provide evidence that protein-protein interactions influence the changes in the time scale and amplitude of protein motion within a domain as well as long-range coupled motions between protein domains [20, 40, 140, 147–149]. Thus, several studies have examined the effect of allostery in PDZ-containing proteins [20, 40, 140, 147–150], and some have shown that allosteric interactions modulate the binding preferences of PDZ domains [20, 40]. Van den Berk et al. (2007) investigated the binding preferences of the 5 PDZ domains in protein tyrosine phosphatase PTP-BL by using a random C-terminal peptide lambda phage display library [40]. They found that the potential of PDZ2 to interact with class III-type ligands can be modulated by the presence of PDZ1. Structural studies have shown that the interaction of PDZ1 with the surface area of PDZ2 opposite the binding groove changes the binding specificity of PDZ2.

Furthermore, Li et al. (2009) reported that the binding of ezrin to NHERF1 increases the binding capabilities of both PDZ domains (Figure 5C) [140]. They further demonstrated that NHERF1 undergoes significant conformational changes in the regions linking PDZ1 and PDZ2 and also those linking PDZ2 and the C-terminal ezrin-binding domain when it forms a complex with ezrin. Together, these results imply that the allosteric behavior in PDZ-mediated protein-protein interactions plays an important role in regulating these interactions.

Deregulation of PDZ-mediated interactions

Consistent with the observations that PDZ protein-protein interactions regulate diverse biological functions, deregulation of PDZ interactions has been linked to various diseases such as cancer [25, 44, 48, 151–155]. Therefore, small molecules, peptides, and peptidomimetics that regulate specific PDZ-mediated interactions have attracted significant attention because of their potential to elicit therapeutic benefits [47, 149, 153, 155–162]. Since these compounds that target specific PDZ-mediated interactions also have a wide-ranging potential as tools for elucidating disease pathways, extensive studies are in progress to discover more potent compounds that inhibit PDZ-mediated interactions [47, 152, 156–165].

Conclusions

Experimental and theoretical studies have been extensively conducted to understand PDZ-mediated interactions. Many studies, however, have used a single PDZ domain despite the presence of multiple copies of the PDZ domain or combination of other interaction modules in proteins. Accumulating studies show, however, that the binding preferences of tandem arrangements within proteins with multiple PDZ domain differ from those of proteins with a single PDZ domain [40, 63, 166–168], implying that careful examination of the binding properties of proteins containing tandem PDZ domains or PDZ domains combined with other interaction module is required. In addition, the biological significance and mechanistic details of multiple PDZ domain-containing proteins still remain to be investigated.

Because PDZ-containing proteins may interact with dozens of proteins, it is paramount to understand the regulatory mechanisms of PDZ protein-protein interactions such as phosphorylation, disulfide bond formation, autoinhibition, competitive binding, and allostery. Phosphorylation of PDZ ligands is likely to be a major regulatory mechanism, but the kinases catalyzing these phosphorylations are often yet to be characterized. We expect that proteomics and bioinformatics can help to determine these kinases and also the phosphorylation sites of the proteins of interest [169–173]. Since other posttranslational modification of proteins such as acetylation have also been proposed [174], future studies also need to focus on identifying and characterizing such unrecognized modifications of PDZ-mediated interactions [175, 176]. An alternative regulatory mechanism that has been proposed for the formation and stabilization of protein complexes is the binding of many PDZ domains to phosphoinositide (PtdInsP)-containing lipid membranes [177–183]. A complete understanding of the regulatory mechanisms of PDZ-mediated interactions will enhance our knowledge of many cellular and biological processes.

Abbreviations

AR:

Alanine Rich region

LZ:

Leucine-Zipper

GPCR:

G Protein Coupled Receptor

NHERF-1:

Na+/H+ Exchanger Regulatory Factor

β2-AR:

β2-adrenergic receptor

GRK-5:

G-protein-coupled receptor kinase

DIX:

Dishevelled and Axin

DEP:

Dishevelled, Egl-10, and Pleckstrin

PKA:

Protein Kinase A

NMDAR:

N-Methyl-D-Aspartate Receptors

NorpA:

No-receptor Potential A

CK2:

Casein Kinase II

PKC:

Protein Kinase C

GRIP:

Glutamate receptor interaction protein

NMDAR:

PKA: Protein Kinase A

LRP4:

Low-density lipoprotein Receptor (LDLR)-related Protein 4

CaMKII:

Ca2+/calmodulin-dependent protein kinase II

HtrA:

High-Temperature Requirement A

SH3:

Src homology 3

GK:

guanylate kinase-like

Y2H:

yeast two-hybrid.

References

  1. Pawson T: Dynamic control of signaling by modular adaptor proteins. Curr Opin Cell Biol. 2007, 19: 112-116. 10.1016/j.ceb.2007.02.013.

    Article  CAS  PubMed  Google Scholar 

  2. Pawson T, Nash P: Assembly of cell regulatory systems through protein interaction domains. Science. 2003, 300: 445-452. 10.1126/science.1083653.

    Article  CAS  PubMed  Google Scholar 

  3. Kennedy MB: Origin of PDZ (DHR, GLGF) domains. Trends Biochem Sci. 1995, 20: 350-10.1016/S0968-0004(00)89074-X.

    Article  CAS  PubMed  Google Scholar 

  4. Doyle DA, Lee A, Lewis J, Kim E, Sheng M, MacKinnon R: Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ. Cell. 1996, 85: 1067-1076. 10.1016/S0092-8674(00)81307-0.

    Article  CAS  PubMed  Google Scholar 

  5. Morais Cabral JH, Petosa C, Sutcliffe MJ, Raza S, Byron O, Poy F, Marfatia SM, Chishti AH, Liddington RC: Crystal structure of a PDZ domain. Nature. 1996, 382: 649-652. 10.1038/382649a0.

    Article  CAS  PubMed  Google Scholar 

  6. Ponting CP: Evidence for PDZ domains in bacteria, yeast, and plants. Protein Sci. 1997, 6: 464-468.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Spaller MR: Act globally, think locally: systems biology addresses the PDZ domain. ACS Chem Biol. 2006, 1: 207-210. 10.1021/cb600191y.

    Article  CAS  PubMed  Google Scholar 

  8. Garner CC, Nash J, Huganir RL: PDZ domains in synapse assembly and signalling. Trends Cell Biol. 2000, 10: 274-280. 10.1016/S0962-8924(00)01783-9.

    Article  CAS  PubMed  Google Scholar 

  9. Sheng M, Sala C: PDZ domains and the organization of supramolecular complexes. Annu Rev Neurosci. 2001, 24: 1-29. 10.1146/annurev.neuro.24.1.1.

    Article  CAS  PubMed  Google Scholar 

  10. Harris BZ, Lim WA: Mechanism and role of PDZ domains in signaling complex assembly. J Cell Sci. 2001, 114: 3219-3231.

    CAS  PubMed  Google Scholar 

  11. Bezprozvanny I, Maximov A: PDZ domains: More than just a glue. Proc Natl Acad Sci USA. 2001, 98: 787-789. 10.1073/pnas.98.3.787.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Fan JS, Zhang M: Signaling complex organization by PDZ domain proteins. Neurosignals. 2002, 11: 315-321. 10.1159/000068256.

    Article  CAS  PubMed  Google Scholar 

  13. Hung AY, Sheng M: PDZ domains: structural modules for protein complex assembly. J Biol Chem. 2002, 277: 5699-5702. 10.1074/jbc.R100065200.

    Article  CAS  PubMed  Google Scholar 

  14. Zhang M, Wang W: Organization of signaling complexes by PDZ-domain scaffold proteins. Acc Chem Res. 2003, 36: 530-538. 10.1021/ar020210b.

    Article  CAS  PubMed  Google Scholar 

  15. van HM, Hendriks W: PDZ domains-glue and guide. Mol Biol Rep. 2003, 30: 69-82. 10.1023/A:1023941703493.

    Article  Google Scholar 

  16. Roh MH, Margolis B: Composition and function of PDZ protein complexes during cell polarization. Am J Physiol Renal Physiol. 2003, 285: F377-F387.

    Article  PubMed  Google Scholar 

  17. Kim DY, Kim KK: Structure and function of HtrA family proteins, the key players in protein quality control. J Biochem Mol Biol. 2005, 38: 266-274.

    Article  CAS  PubMed  Google Scholar 

  18. Brone B, Eggermont J: PDZ proteins retain and regulate membrane transporters in polarized epithelial cell membranes. Am J Physiol Cell Physiol. 2005, 288: C20-C29.

    Article  CAS  PubMed  Google Scholar 

  19. Dev KK: PDZ domain protein-protein interactions: a case study with PICK1. Curr Top Med Chem. 2007, 7: 3-20. 10.2174/156802607779318343.

    Article  CAS  PubMed  Google Scholar 

  20. Petit CM, Zhang J, Sapienza PJ, Fuentes EJ, Lee AL: Hidden dynamic allostery in a PDZ domain. Proc Natl Acad Sci USA. 2009, 106: 18249-18254. 10.1073/pnas.0904492106.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Fanning AS, Anderson JM: Protein-protein interactions: PDZ domain networks. Curr Biol. 1996, 6: 1385-1388. 10.1016/S0960-9822(96)00737-3.

    Article  CAS  PubMed  Google Scholar 

  22. Saras J, Heldin CH: PDZ domains bind carboxy-terminal sequences of target proteins. Trends Biochem Sci. 1996, 21: 455-458. 10.1016/S0968-0004(96)30044-3.

    Article  CAS  PubMed  Google Scholar 

  23. Cowburn D: Peptide recognition by PTB and PDZ domains. Curr Opin Struct Biol. 1997, 7: 835-838. 10.1016/S0959-440X(97)80155-8.

    Article  CAS  PubMed  Google Scholar 

  24. Giallourakis C, Cao Z, Green T, Wachtel H, Xie X, Lopez-Illasaca M, Daly M, Rioux J, Xavier R: A molecular-properties-based approach to understanding PDZ domain proteins and PDZ ligands. Genome Res. 2006, 16: 1056-1072. 10.1101/gr.5285206.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  25. Wang NX, Lee HJ, Zheng JJ: Therapeutic use of PDZ protein-protein interaction antagonism. Drug News Perspect. 2008, 21: 137-141.

    PubMed Central  CAS  PubMed  Google Scholar 

  26. Laskowski RA, Chistyakov VV, Thornton JM: PDBsum more: new summaries and analyses of the known 3D structures of proteins and nucleic acids. Nucleic Acids Res. 2005, 33: D266-D268. 10.1093/nar/gki001.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  27. Basdevant N, Weinstein H, Ceruso M: Thermodynamic basis for promiscuity and selectivity in protein-protein interactions: PDZ domains, a case study. J Am Chem Soc. 2006, 128: 12766-12777. 10.1021/ja060830y.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  28. Fam SR, Paquet M, Castleberry AM, Oller H, Lee CJ, Traynelis SF, Smith Y, Yun CC, Hall RA: P2Y1 receptor signaling is controlled by interaction with the PDZ scaffold NHERF-2. Proc Natl Acad Sci USA. 2005, 102: 8042-8047. 10.1073/pnas.0408818102.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Paquet M, Asay MJ, Fam SR, Inuzuka H, Castleberry AM, Oller H, Smith Y, Yun CC, Traynelis SF, Hall RA: The PDZ scaffold NHERF-2 interacts with mGluR5 and regulates receptor activity. J Biol Chem. 2006, 281: 29949-29961. 10.1074/jbc.M602262200.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. He J, Bellini M, Inuzuka H, Xu J, Xiong Y, Yang X, Castleberry AM, Hall RA: Proteomic analysis of beta1-adrenergic receptor interactions with PDZ scaffold proteins. J Biol Chem. 2006, 281: 2820-2827. 10.1074/jbc.M509503200.

    Article  CAS  PubMed  Google Scholar 

  31. Songyang Z, Fanning AS, Fu C, Xu J, Marfatia SM, Chishti AH, Crompton A, Chan AC, Anderson JM, Cantley LC: Recognition of unique carboxyl-terminal motifs by distinct PDZ domains. Science. 1997, 275: 73-77. 10.1126/science.275.5296.73.

    Article  CAS  PubMed  Google Scholar 

  32. Fuh G, Pisabarro MT, Li Y, Quan C, Lasky LA, Sidhu SS: Analysis of PDZ domain-ligand interactions using carboxyl-terminal phage display. J Biol Chem. 2000, 275: 21486-21491.

    CAS  PubMed  Google Scholar 

  33. Sidhu SS: Engineering M13 for phage display. Biomol Eng. 2001, 18: 57-63. 10.1016/S1389-0344(01)00087-9.

    Article  CAS  PubMed  Google Scholar 

  34. Kurakin A, Bredesen D: Target-assisted iterative screening reveals novel interactors for PSD95, Nedd4, Src, Abl and Crk proteins. J Biomol Struct Dyn. 2002, 19: 1015-1029.

    Article  CAS  PubMed  Google Scholar 

  35. Sidhu SS, Fairbrother WJ, Deshayes K: Exploring protein-protein interactions with phage display. Chembiochem. 2003, 4: 14-25. 10.1002/cbic.200390008.

    Article  CAS  PubMed  Google Scholar 

  36. Appleton BA, Zhang Y, Wu P, Yin JP, Hunziker W, Skelton NJ, Sidhu SS, Wiesmann C: Comparative structural analysis of the Erbin PDZ domain and the first PDZ domain of ZO-1. Insights into determinants of PDZ domain specificity. J Biol Chem. 2006, 281: 22312-22320. 10.1074/jbc.M602901200.

    Article  CAS  PubMed  Google Scholar 

  37. Sidhu SS, Koide S: Phage display for engineering and analyzing protein interaction interfaces. Curr Opin Struct Biol. 2007, 17: 481-487. 10.1016/j.sbi.2007.08.007.

    Article  CAS  PubMed  Google Scholar 

  38. Boisguerin P, Ay B, Radziwill G, Fritz RD, Moelling K, Volkmer R: Characterization of a Putative Phosphorylation Switch: Adaptation of SPOT Synthesis to Analyze PDZ Domain Regulation Mechanisms. Chembiochem. 2007

    Google Scholar 

  39. Kurakin A, Swistowski A, Wu SC, Bredesen DE: The PDZ domain as a complex adaptive system. PLoS ONE. 2007, 2: e953-10.1371/journal.pone.0000953.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  40. Berk van den LC, Landi E, Walma T, Vuister GW, Dente L, Hendriks WJ: An allosteric intramolecular PDZ-PDZ interaction modulates PTP-BL PDZ2 binding specificity. Biochemistry. 2007, 46: 13629-13637. 10.1021/bi700954e.

    Article  PubMed  CAS  Google Scholar 

  41. Runyon ST, Zhang Y, Appleton BA, Sazinsky SL, Wu P, Pan B, Wiesmann C, Skelton NJ, Sidhu SS: Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Sci. 2007, 16: 2454-2471. 10.1110/ps.073049407.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  42. Zhang Y, Appleton BA, Wu P, Wiesmann C, Sidhu SS: Structural and functional analysis of the ligand specificity of the HtrA2/Omi PDZ domain. Protein Sci. 2007, 16: 1738-1750. 10.1110/ps.072833207.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  43. Joo SH, Pei D: Synthesis and screening of support-bound combinatorial Peptide libraries with free C-termini: determination of the sequence specificity of PDZ domains. Biochemistry. 2008, 47: 3061-3072. 10.1021/bi7023628.

    Article  CAS  PubMed  Google Scholar 

  44. Cushing PR, Fellows A, Villone D, Boisguerin P, Madden DR: The relative binding affinities of PDZ partners for CFTR: a biochemical basis for efficient endocytic recycling. Biochemistry. 2008, 47: 10084-10098. 10.1021/bi8003928.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  45. Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM: NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Mol Cell Biol. 2007, 27: 2527-2537. 10.1128/MCB.01372-06.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  46. Lee H-J, Wang NX, Shao Y, Zheng JJ: Identification of tripeptides recognized by the PDZ domain of Dishevelled. Bioorg Med Chem. 2009, 17: 1701-1708. 10.1016/j.bmc.2008.12.060.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  47. Lee HJ, Wang NX, Shi DL, Zheng JJ: Sulindac inhibits canonical Wnt signaling by blocking the PDZ domain of the protein Dishevelled. Angew Chem Int Ed Engl. 2009, 48: 6448-6452. 10.1002/anie.200902981.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  48. Wong HC, Bourdelas A, Krauss A, Lee HJ, Shao Y, Wu D, Mlodzik M, Shi DL, Zheng J: Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled. Mol Cell. 2003, 12: 1251-1260. 10.1016/S1097-2765(03)00427-1.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  49. Zhang Y, Appleton BA, Wiesmann C, Lau T, Costa M, Hannoush RN, Sidhu SS: Inhibition of Wnt signaling by Dishevelled PDZ peptides. Nat Chem Biol. 2009, 5: 217-219. 10.1038/nchembio.152.

    Article  CAS  PubMed  Google Scholar 

  50. Im YJ, Lee JH, Park SH, Park SJ, Rho SH, Kang GB, Kim E, Eom SH: Crystal structure of the Shank PDZ-ligand complex reveals a class I PDZ interaction and a novel PDZ-PDZ dimerization. J Biol Chem. 2003, 278: 48099-48104. 10.1074/jbc.M306919200.

    Article  CAS  PubMed  Google Scholar 

  51. Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH: Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization. J Biol Chem. 2003, 278: 8501-8507. 10.1074/jbc.M212263200.

    Article  CAS  PubMed  Google Scholar 

  52. Utepbergenov DI, Fanning AS, Anderson JM: Dimerization of the scaffolding protein ZO-1 through the second PDZ domain. J Biol Chem. 2006, 281: 24671-24677. 10.1074/jbc.M512820200.

    Article  CAS  PubMed  Google Scholar 

  53. Wu J, Yang Y, Zhang J, Ji P, Du W, Jiang P, Xie D, Huang H, Wu M, Zhang G, Wu J, Shi Y: Domain-swapped dimerization of the second PDZ domain of ZO2 may provide a structural basis for the polymerization of claudins. J Biol Chem. 2007

    Google Scholar 

  54. Fanning AS, Lye MF, Anderson JM, Arnon L: Domain swapping within PDZ2 is responsible for dimerization of ZO proteins. J Biol Chem. 2007

    Google Scholar 

  55. Chen J, Pan L, Wei Z, Zhao Y, Zhang M: Domain-swapped dimerization of ZO-1 PDZ2 generates specific and regulatory connexin43-binding sites. EMBO J. 2008, 27: 2113-2123. 10.1038/emboj.2008.138.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  56. Long J, Wei Z, Feng W, Yu C, Zhao YX, Zhang M: Supramodular nature of GRIP1 revealed by the structure of its PDZ12 tandem in complex with the carboxyl tail of Fras1. J Mol Biol. 2008, 375: 1457-1468. 10.1016/j.jmb.2007.11.088.

    Article  CAS  PubMed  Google Scholar 

  57. Feng W, Shi Y, Li M, Zhang M: Tandem PDZ repeats in glutamate receptor-interacting proteins have a novel mode of PDZ domain-mediated target binding. Nat Struct Biol. 2003, 10: 972-978. 10.1038/nsb992.

    Article  CAS  PubMed  Google Scholar 

  58. Long JF, Tochio H, Wang P, Fan JS, Sala C, Niethammer M, Sheng M, Zhang M: Supramodular structure and synergistic target binding of the N-terminal tandem PDZ domains of PSD-95. J Mol Biol. 2003, 327: 203-214. 10.1016/S0022-2836(03)00113-X.

    Article  CAS  PubMed  Google Scholar 

  59. Kang BS, Cooper DR, Jelen F, Devedjiev Y, Derewenda U, Dauter Z, Otlewski J, Derewenda ZS: PDZ tandem of human syntenin: crystal structure and functional properties. Structure. 2003, 11: 459-468. 10.1016/S0969-2126(03)00052-2.

    Article  CAS  PubMed  Google Scholar 

  60. Grembecka J, Cierpicki T, Devedjiev Y, Derewenda U, Kang BS, Bushweller JH, Derewenda ZS: The binding of the PDZ tandem of syntenin to target proteins. Biochemistry. 2006, 45: 3674-3683. 10.1021/bi052225y.

    Article  CAS  PubMed  Google Scholar 

  61. Long JF, Feng W, Wang R, Chan LN, Ip FC, Xia J, Ip NY, Zhang M: Autoinhibition of X11/Mint scaffold proteins revealed by the closed conformation of the PDZ tandem. Nat Struct Mol Biol. 2005, 12: 722-728. 10.1038/nsmb958.

    Article  CAS  PubMed  Google Scholar 

  62. Dong H, O'Brien RJ, Fung ET, Lanahan AA, Worley PF, Huganir RL: GRIP: a synaptic PDZ domain-containing protein that interacts with AMPA receptors. Nature. 1997, 386: 279-284. 10.1038/386279a0.

    Article  CAS  PubMed  Google Scholar 

  63. Feng W, Zhang M: Organization and dynamics of PDZ-domain-related supramodules in the postsynaptic density. Nat Rev Neurosci. 2009, 10: 87-99. 10.1038/nrn2540.

    Article  CAS  PubMed  Google Scholar 

  64. Karplus PA: Experimentally observed conformation-dependent geometry and hidden strain in proteins. Protein Sci. 1996, 5: 1406-1420. 10.1002/pro.5560050719.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  65. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT: Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Dev Cell. 2002, 2: 449-461. 10.1016/S1534-5807(02)00140-5.

    Article  CAS  PubMed  Google Scholar 

  66. Hillier BJ, Christopherson KS, Prehoda KE, Bredt DS, Lim WA: Unexpected modes of PDZ domain scaffolding revealed by structure of nNOS-syntrophin complex. Science. 1999, 284: 812-815. 10.1126/science.284.5415.812.

    Article  CAS  PubMed  Google Scholar 

  67. Brenman JE, Chao DS, Gee SH, McGee AW, Craven SE, Santillano DR, Wu Z, Huang F, Xia H, Peters MF, Froehner SC, Bredt DS: Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains. Cell. 1996, 84: 757-767. 10.1016/S0092-8674(00)81053-3.

    Article  CAS  PubMed  Google Scholar 

  68. Gee SH, Sekely SA, Lombardo C, Kurakin A, Froehner SC, Kay BK: Cyclic peptides as non-carboxyl-terminal ligands of syntrophin PDZ domains. J Biol Chem. 1998, 273: 21980-21987. 10.1074/jbc.273.34.21980.

    Article  CAS  PubMed  Google Scholar 

  69. Penkert RR, DiVittorio HM, Prehoda KE: Internal recognition through PDZ domain plasticity in the Par-6-Pals1 complex. Nat Struct Mol Biol. 2004, 11: 1122-1127. 10.1038/nsmb839.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  70. London TB, Lee HJ, Shao Y, Zheng J: Interaction between the internal motif KTXXXI of Idax and mDvl PDZ domain. Biochem Biophys Res Commun. 2004, 322: 326-332. 10.1016/j.bbrc.2004.07.113.

    Article  CAS  PubMed  Google Scholar 

  71. Skelton NJ, Koehler MF, Zobel K, Wong WL, Yeh S, Pisabarro MT, Yin JP, Lasky LA, Sidhu SS: Origins of PDZ domain ligand specificity. Structure determination and mutagenesis of the Erbin PDZ domain. J Biol Chem. 2003, 278: 7645-7654. 10.1074/jbc.M209751200.

    Article  CAS  PubMed  Google Scholar 

  72. Kang BS, Cooper DR, Devedjiev Y, Derewenda U, Derewenda ZS: Molecular roots of degenerate specificity in syntenin's PDZ2 domain: reassessment of the PDZ recognition paradigm. Structure. 2003, 11: 845-853. 10.1016/S0969-2126(03)00125-4.

    Article  CAS  PubMed  Google Scholar 

  73. Tonikian R, Zhang Y, Sazinsky SL, Currell B, Yeh JH, Reva B, Held HA, Appleton BA, Evangelista M, Wu Y, Xin X, Chan AC, Seshagiri S, Lasky LA, Sander C, Boone C, Bader GD, Sidhu SS: A specificity map for the PDZ domain family. PLoS Biol. 2008, 6: e239-10.1371/journal.pbio.0060239.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  74. Tonikian R, Zhang YN, Boone C, Sidhu SS: Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries. Nature Protocols. 2007, 2: 1368-1386. 10.1038/nprot.2007.151.

    Article  CAS  PubMed  Google Scholar 

  75. Zhang Y, Yeh S, Appleton BA, Held HA, Kausalya PJ, Phua DC, Wong WL, Lasky LA, Wiesmann C, Hunziker W, Sidhu SS: Convergent and divergent ligand specificity among PDZ domains of the LAP and zonula occludens (ZO) families. J Biol Chem. 2006, 281: 22299-22311. 10.1074/jbc.M602902200.

    Article  CAS  PubMed  Google Scholar 

  76. Beuming T, Farid R, Sherman W: High-energy water sites determine peptide binding affinity and specificity of PDZ domains. Protein Sci. 2009, 18: 1609-1619. 10.1002/pro.177.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  77. Kimple ME, Siderovski DP, Sondek J: Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA. EMBO J. 2001, 20: 4414-4422. 10.1093/emboj/20.16.4414.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  78. Jelen F, Oleksy A, Smietana K, Otlewski J: PDZ domains - common players in the cell signaling. Acta Biochim Pol. 2003, 50: 985-1017.

    CAS  PubMed  Google Scholar 

  79. Song E, Gao S, Tian R, Ma S, Huang H, Guo J, Li Y, Zhang L, Gao Y: A high efficiency strategy for binding property characterization of peptide-binding domains. Mol Cell Proteomics. 2006, 5: 1368-1381. 10.1074/mcp.M600072-MCP200.

    Article  CAS  PubMed  Google Scholar 

  80. Piserchio A, Spaller M, Mierke DF: Targeting the PDZ domains of molecular scaffolds of transmembrane ion channels. AAPS J. 2006, 8: E396-E401.

    PubMed Central  PubMed  Google Scholar 

  81. Hering H, Sheng M: Direct interaction of Frizzled-1, -2, -4, and -7 with PDZ domains of PSD-95. FEBS Lett. 2002, 521: 185-189. 10.1016/S0014-5793(02)02831-4.

    Article  CAS  PubMed  Google Scholar 

  82. Uetz P: Two-hybrid arrays. Curr Opin Chem Biol. 2002, 6: 57-62. 10.1016/S1367-5931(01)00288-5.

    Article  CAS  PubMed  Google Scholar 

  83. Soares HD, Williams SA, Snyder PJ, Gao F, Stiger T, Rohlff C, Herath A, Sunderland T, Putnam K, White WF: Proteomic approaches in drug discovery and development. Int Rev Neurobiol. 2004, 61: 97-126. full_text.

    Article  CAS  PubMed  Google Scholar 

  84. Lee HW, Ko J, Kim E: Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays. Methods Mol Biol. 2006, 332: 233-244.

    CAS  PubMed  Google Scholar 

  85. Gisler SM, Kittanakom S, Fuster D, Wong V, Bertic M, Radanovic T, Hall RA, Murer H, Biber J, Markovich D, Moe OW, Stagljar I: Monitoring protein-protein interactions between the mammalian integral membrane transporters and PDZ-interacting partners using a modified split-ubiquitin membrane yeast two-hybrid system. Mol Cell Proteomics. 2008, 7: 1362-1377. 10.1074/mcp.M800079-MCP200.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  86. von NP, Ismail M, Gardin C, Suila H, Zara I, Belgrano A, Valle G, Carpen O, Faulkner G: A class III PDZ binding motif in the myotilin and FATZ families binds enigma family proteins: a common link for Z-disc myopathies. Mol Cell Biol. 2009, 29: 822-834. 10.1128/MCB.01454-08.

    Article  CAS  Google Scholar 

  87. Wolf-Yadlin A, Sevecka M, MacBeath G: Dissecting protein function and signaling using protein microarrays. Curr Opin Chem Biol. 2009, 13: 398-405. 10.1016/j.cbpa.2009.06.027.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  88. Poetz O, Luckert K, Herget T, Joos TO: Microsphere-based co-immunoprecipitation in multiplex. Anal Biochem. 2009, 395: 244-248. 10.1016/j.ab.2009.08.002.

    Article  CAS  PubMed  Google Scholar 

  89. Lee HW, Choi J, Shin H, Kim K, Yang J, Na M, Choi SY, Kang GB, Eom SH, Kim H, Kim E: Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis. J Neurosci. 2008, 28: 14546-14556. 10.1523/JNEUROSCI.3112-08.2008.

    Article  CAS  PubMed  Google Scholar 

  90. Zhang J, Cheng S, Xiong Y, Ma Y, Luo D, Jeromin A, Zhang H, He J: A novel association of mGluR1a with the PDZ scaffold protein CAL modulates receptor activity. FEBS Lett. 2008, 582: 4117-4124. 10.1016/j.febslet.2008.10.054.

    Article  CAS  PubMed  Google Scholar 

  91. Meerschaert K, Remue E, De GA, Staes A, Boucherie C, Gevaert K, Vandekerckhove J, Kleiman L, Gettemans J: The tandem PDZ protein Syntenin interacts with the aminoacyl tRNA synthetase complex in a lysyl-tRNA synthetase-dependent manner. J Proteome Res. 2008, 7: 4962-4973. 10.1021/pr800325u.

    Article  CAS  PubMed  Google Scholar 

  92. Gee HY, Kim YW, Jo MJ, Namkung W, Kim JY, Park HW, Kim KS, Kim H, Baba A, Yang J, Kim E, Kim KH, Lee MG: Synaptic scaffolding molecule binds to and regulates vasoactive intestinal polypeptide type-1 receptor in epithelial cells. Gastroenterology. 2009, 137: 607-17. 10.1053/j.gastro.2009.01.065. 617.

    Article  CAS  PubMed  Google Scholar 

  93. Terpe K: Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 2003, 60: 523-533.

    Article  CAS  PubMed  Google Scholar 

  94. Cui H, Hayashi A, Sun HS, Belmares MP, Cobey C, Phan T, Schweizer J, Salter MW, Wang YT, Tasker RA, Garman D, Rabinowitz J, Lu PS, Tymianski M: PDZ protein interactions underlying NMDA receptor-mediated excitotoxicity and neuroprotection by PSD-95 inhibitors. J Neurosci. 2007, 27: 9901-9915. 10.1523/JNEUROSCI.1464-07.2007.

    Article  CAS  PubMed  Google Scholar 

  95. Obenauer JC, Denson J, Mehta PK, Su X, Mukatira S, Finkelstein DB, Xu X, Wang J, Ma J, Fan Y, Rakestraw KM, Webster RG, Hoffmann E, Krauss S, Zheng J, Zhang Z, Naeve CW: Large-scale sequence analysis of avian influenza isolates. Science. 2006, 311: 1576-1580. 10.1126/science.1121586.

    Article  CAS  PubMed  Google Scholar 

  96. Jackson D, Hossain MJ, Hickman D, Perez DR, Lamb RA: A new influenza virus virulence determinant: The NS1 protein four C-terminal residues modulate pathogenicity. Proc Natl Acad Sci USA. 2008, 105: 4381-4386. 10.1073/pnas.0800482105.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  97. Stiffler MA, Chen JR, Grantcharova VP, Lei Y, Fuchs D, Allen JE, Zaslavskaia LA, MacBeath G: PDZ domain binding selectivity is optimized across the mouse proteome. Science. 2007, 317: 364-369. 10.1126/science.1144592.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  98. Stiffler MA, Grantcharova VP, Sevecka M, MacBeath G: Uncovering quantitative protein interaction networks for mouse PDZ domains using protein microarrays. J Am Chem Soc. 2006, 128: 5913-5922. 10.1021/ja060943h.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  99. Chen JR, Chang BH, Allen JE, Stiffler MA, MacBeath G: Predicting PDZ domain-peptide interactions from primary sequences. Nat Biotechnol. 2008, 26: 1041-1045. 10.1038/nbt.1489.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  100. Song E, Gao S, Tian R, Ma S, Huang H, Guo J, Li Y, Zhang L, Gao Y: A high efficiency strategy for binding property characterization of peptide-binding domains. Mol Cell Proteomics. 2006, 5: 1368-1381. 10.1074/mcp.M600072-MCP200.

    Article  CAS  PubMed  Google Scholar 

  101. Sidhu SS, Bader GD, Boone C: Functional genomics of intracellular peptide recognition domains with combinatorial biology methods. Curr Opin Chem Biol. 2003, 7: 97-102. 10.1016/S1367-5931(02)00011-X.

    Article  CAS  PubMed  Google Scholar 

  102. Berk van den LC, Landi E, Harmsen E, Dente L, Hendriks WJ: Redox-regulated affinity of the third PDZ domain in the phosphotyrosine phosphatase PTP-BL for cysteine-containing target peptides. FEBS J. 2005, 272: 3306-3316. 10.1111/j.1742-4658.2005.04743.x.

    Article  PubMed  CAS  Google Scholar 

  103. Wiedemann U, Boisguerin P, Leben R, Leitner D, Krause G, Moelling K, Volkmer-Engert R, Oschkinat H: Quantification of PDZ domain specificity, prediction of ligand affinity and rational design of super-binding peptides. J Mol Biol. 2004, 343: 703-718. 10.1016/j.jmb.2004.08.064.

    Article  CAS  PubMed  Google Scholar 

  104. Lemaire JF, McPherson PS: Binding of Vac14 to neuronal nitric oxide synthase: Characterisation of a new internal PDZ-recognition motif. FEBS Lett. 2006, 580: 6948-6954. 10.1016/j.febslet.2006.11.061.

    Article  CAS  PubMed  Google Scholar 

  105. Mathivanan S, Periaswamy B, Gandhi TK, Kandasamy K, Suresh S, Mohmood R, Ramachandra YL, Pandey A: An evaluation of human protein-protein interaction data in the public domain. BMC Bioinformatics. 2006, 7 (Suppl 5): S19-10.1186/1471-2105-7-S5-S19.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  106. Ceol A, Chatr-Aryamontri A, Santonico E, Sacco R, Castagnoli L, Cesareni G: DOMINO: a database of domain-peptide interactions. Nucleic Acids Res. 2007, 35: D557-D560. 10.1093/nar/gkl961.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  107. Brannetti B, Helmer-Citterich M: iSPOT: A web tool to infer the interaction specificity of families of protein modules. Nucleic Acids Res. 2003, 31: 3709-3711. 10.1093/nar/gkg592.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  108. Beuming T, Skrabanek L, Niv MY, Mukherjee P, Weinstein H: PDZBase: a protein-protein interaction database for PDZ-domains. Bioinformatics. 2005, 21: 827-828. 10.1093/bioinformatics/bti098.

    Article  CAS  PubMed  Google Scholar 

  109. Wallingford JB, Habas R: The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity. Development. 2005, 132: 4421-4436. 10.1242/dev.02068.

    Article  CAS  PubMed  Google Scholar 

  110. Kobayashi H, Michiue T, Yukita A, Danno H, Sakurai K, Fukui A, Kikuchi A, Asashima M: Novel Daple-like protein positively regulates both the Wnt/beta-catenin pathway and the Wnt/JNK pathway in Xenopus. Mech Dev. 2005, 122: 1138-1153. 10.1016/j.mod.2005.05.003.

    Article  CAS  PubMed  Google Scholar 

  111. Schillinger C, Boisguerin P, Krause G: Domain Interaction Footprint: a multi-classification approach to predict domain-peptide interactions. Bioinformatics. 2009, 25: 1632-1639. 10.1093/bioinformatics/btp264.

    Article  CAS  PubMed  Google Scholar 

  112. Chung HJ, Huang YH, Lau LF, Huganir RL: Regulation of the NMDA receptor complex and trafficking by activity-dependent phosphorylation of the NR2B subunit PDZ ligand. J Neurosci. 2004, 24: 10248-10259. 10.1523/JNEUROSCI.0546-04.2004.

    Article  CAS  PubMed  Google Scholar 

  113. Girault JA, Hemmings HC, Zorn SH, Gustafson EL, Greengard P: Characterization in mammalian brain of a DARPP-32 serine kinase identical to casein kinase II. J Neurochem. 1990, 55: 1772-1783. 10.1111/j.1471-4159.1990.tb04968.x.

    Article  CAS  PubMed  Google Scholar 

  114. Chetkovich DM, Chen L, Stocker TJ, Nicoll RA, Bredt DS: Phosphorylation of the postsynaptic density-95 (PSD-95)/discs large/zona occludens-1 binding site of stargazin regulates binding to PSD-95 and synaptic targeting of AMPA receptors. J Neurosci. 2002, 22: 5791-5796.

    CAS  PubMed  Google Scholar 

  115. Choi J, Ko J, Park E, Lee JR, Yoon J, Lim S, Kim E: Phosphorylation of stargazin by protein kinase A regulates its interaction with PSD-95. J Biol Chem. 2002, 277: 12359-12363. 10.1074/jbc.M200528200.

    Article  CAS  PubMed  Google Scholar 

  116. Matsuda S, Mikawa S, Hirai H: Phosphorylation of serine-880 in GluR2 by protein kinase C prevents its C terminus from binding with glutamate receptor-interacting protein. J Neurochem. 1999, 73: 1765-1768. 10.1046/j.1471-4159.1999.731765.x.

    Article  CAS  PubMed  Google Scholar 

  117. Chung HJ, Xia J, Scannevin RH, Zhang X, Huganir RL: Phosphorylation of the AMPA receptor subunit GluR2 differentially regulates its interaction with PDZ domain-containing proteins. J Neurosci. 2000, 20: 7258-7267.

    CAS  PubMed  Google Scholar 

  118. Lin DT, Huganir RL: PICK1 and phosphorylation of the glutamate receptor 2 (GluR2) AMPA receptor subunit regulates GluR2 recycling after NMDA receptor-induced internalization. J Neurosci. 2007, 27: 13903-13908. 10.1523/JNEUROSCI.1750-07.2007.

    Article  CAS  PubMed  Google Scholar 

  119. Tian QB, Suzuki T, Yamauchi T, Sakagami H, Yoshimura Y, Miyazawa S, Nakayama K, Saitoh F, Zhang JP, Lu Y, Kondo H, Endo S: Interaction of LDL receptor-related protein 4 (LRP4) with postsynaptic scaffold proteins via its C-terminal PDZ domain-binding motif, and its regulation by Ca/calmodulin-dependent protein kinase II. Eur J Neurosci. 2006, 23: 2864-2876. 10.1111/j.1460-9568.2006.04846.x.

    Article  PubMed  Google Scholar 

  120. Park DJ, Wallick CJ, Martyn KD, Lau AF, Jin C, Warn-Cramer BJ: Akt phosphorylates connexin43 on Ser373, a "Mode-1" binding site for 14-3-3. Cell Communication and Adhesion. 2007, 14: 211-226. 10.1080/15419060701755958.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  121. Solan JL, Fry MD, TenBroek EM, Lampe PD: Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation. J Cell Sci. 2003, 116: 2203-2211. 10.1242/jcs.00428.

    Article  CAS  PubMed  Google Scholar 

  122. Solan JL, Lampe PD: Connexin phosphorylation as a regulatory event linked to gap junction channel assembly. Biochim Biophys Acta. 2005, 1711: 154-163. 10.1016/j.bbamem.2004.09.013.

    Article  CAS  PubMed  Google Scholar 

  123. Madoz-Gurpide J, Canamero M, Sanchez L, Solano J, Alfonso P, Casal JI: A proteomic analysis of cell signaling alterations in colorectal cancer. Mol Cell Proteomics. 2007

    Google Scholar 

  124. Solan JL, Marquez-Rosado L, Sorgen PL, Thornton PJ, Gafken PR, Lampe PD: Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC. J Cell Biol. 2007, 179: 1301-1309. 10.1083/jcb.200707060.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  125. Solan JL, Lampe PD: Connexin43 phosphorylation: structural changes and biological effects. Biochem J. 2009, 419: 261-272. 10.1042/BJ20082319.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  126. Adey NB, Huang L, Ormonde PA, Baumgard ML, Pero R, Byreddy DV, Tavtigian SV, Bartel PL: Threonine phosphorylation of the MMAC1/PTEN PDZ binding domain both inhibits and stimulates PDZ binding. Cancer Res. 2000, 60: 35-37.

    CAS  PubMed  Google Scholar 

  127. Chen BS, Braud S, Badger JD, Isaac JT, Roche KW: Regulation of NR1/NR2C N-methyl-D-aspartate (NMDA) receptors by phosphorylation. J Biol Chem. 2006, 281: 16583-16590. 10.1074/jbc.M513029200.

    Article  CAS  PubMed  Google Scholar 

  128. Mishra P, Socolich M, Wall MA, Graves J, Wang Z, Ranganathan R: Dynamic scaffolding in a g protein-coupled signaling system. Cell. 2007, 131: 80-92. 10.1016/j.cell.2007.07.037.

    Article  CAS  PubMed  Google Scholar 

  129. Montell C: Dynamic regulation of the INAD signaling scaffold becomes crystal clear. Cell. 2007, 131: 19-21. 10.1016/j.cell.2007.09.022.

    Article  CAS  PubMed  Google Scholar 

  130. Mauceri D, Gardoni F, Marcello E, Di LM: Dual role of CaMKII-dependent SAP97 phosphorylation in mediating trafficking and insertion of NMDA receptor subunit NR2A. J Neurochem. 2007, 100: 1032-1046. 10.1111/j.1471-4159.2006.04267.x.

    Article  CAS  PubMed  Google Scholar 

  131. Steiner P, Higley MJ, Xu W, Czervionke BL, Malenka RC, Sabatini BL: Destabilization of the postsynaptic density by PSD-95 serine 73 phosphorylation inhibits spine growth and synaptic plasticity. Neuron. 2008, 60: 788-802. 10.1016/j.neuron.2008.10.014.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  132. Seidler U, Singh AK, Cinar A, Chen M, Hillesheim J, Hogema B, Riederer B: The role of the NHERF family of PDZ scaffolding proteins in the regulation of salt and water transport. Ann N Y Acad Sci. 2009, 1165: 249-260. 10.1111/j.1749-6632.2009.04046.x.

    Article  CAS  PubMed  Google Scholar 

  133. Voltz JW, Brush M, Sikes S, Steplock D, Weinman EJ, Shenolikar S: Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets. J Biol Chem. 2007, 282: 33879-33887. 10.1074/jbc.M703481200.

    Article  CAS  PubMed  Google Scholar 

  134. Weinman EJ, Biswas RS, Peng Q, Shen L, Turner CLEX, Steplock D, Shenolikar S, Cunningham R: Parathyroid hormone inhibits renal phosphate transport by phosphorylation of serine 77 of sodium-hydrogen exchanger regulatory factor-1. J Clin Invest. 2007, 117: 3412-3420. 10.1172/JCI32738.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  135. Raghuram V, Hormuth H, Foskett JK: A kinase-regulated mechanism controls CFTR channel gating by disrupting bivalent PDZ domain interactions. Proc Natl Acad Sci USA. 2003, 100: 9620-9625. 10.1073/pnas.1633250100.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  136. Li J, Poulikakos PI, Dai Z, Testa JR, Callaway DJ, Bu Z: Protein kinase C phosphorylation disrupts Na+/H+ exchanger regulatory factor 1 autoinhibition and promotes cystic fibrosis transmembrane conductance regulator macromolecular assembly. J Biol Chem. 2007, 282: 27086-27099. 10.1074/jbc.M702019200.

    Article  CAS  PubMed  Google Scholar 

  137. Lalonde D, Bretscher A: The scaffold protein PDZK1 undergoes a head-to-tail intramolecular association that negatively regulates its interaction with EBP50. Biochemistry. 2009

    Google Scholar 

  138. Terawaki S, Maesaki R, Okada K, Hakoshima T: Crystallographic characterization of the radixin FERM domain bound to the C-terminal region of the human Na+/H+-exchanger regulatory factor (NHERF). Acta Crystallogr D Biol Crystallogr. 2003, 59: 177-179. 10.1107/S0907444902019686.

    Article  PubMed  CAS  Google Scholar 

  139. Cheng H, Li J, Fazlieva R, Dai Z, Bu Z, Roder H: Autoinhibitory interactions between the PDZ2 and C-terminal domains in the scaffolding protein NHERF1. Structure. 2009, 17: 660-669. 10.1016/j.str.2009.03.009.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  140. Li J, Callaway DJ, Bu Z: Ezrin induces long-range interdomain allostery in the scaffolding protein NHERF1. J Mol Biol. 2009, 392: 166-180. 10.1016/j.jmb.2009.07.005.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  141. Sugi T, Oyama T, Muto T, Nakanishi S, Morikawa K, Jingami H: Crystal structures of autoinhibitory PDZ domain of Tamalin: implications for metabotropic glutamate receptor trafficking regulation. EMBO J. 2007, 26: 2192-2205. 10.1038/sj.emboj.7601651.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  142. Rogelj B, Mitchell JC, Miller CC, McLoughlin DM: The X11/Mint family of adaptor proteins. Brain Res Rev. 2006, 52: 305-315. 10.1016/j.brainresrev.2006.04.005.

    Article  CAS  PubMed  Google Scholar 

  143. Nevrivy DJ, Peterson VJ, Avram D, Ishmael JE, Hansen SG, Dowell P, Hruby DE, Dawson MI, Leid M: Interaction of GRASP, a protein encoded by a novel retinoic acid-induced gene, with members of the cytohesin family of guanine nucleotide exchange factors. J Biol Chem. 2000, 275: 16827-16836. 10.1074/jbc.275.22.16827.

    Article  CAS  PubMed  Google Scholar 

  144. Kitano J, Kimura K, Yamazaki Y, Soda T, Shigemoto R, Nakajima Y, Nakanishi S: Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins. J Neurosci. 2002, 22: 1280-1289.

    CAS  PubMed  Google Scholar 

  145. Kitano J, Yamazaki Y, Kimura K, Masukado T, Nakajima Y, Nakanishi S: Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association. J Biol Chem. 2003, 278: 14762-14768. 10.1074/jbc.M300184200.

    Article  CAS  PubMed  Google Scholar 

  146. Hirose M, Kitano J, Nakajima Y, Moriyoshi K, Yanagi S, Yamamura H, Muto T, Jingami H, Nakanishi S: Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin. J Biol Chem. 2004, 279: 32308-32315. 10.1074/jbc.M400547200.

    Article  CAS  PubMed  Google Scholar 

  147. Fuentes EJ, Der CJ, Lee AL: Ligand-dependent dynamics and intramolecular signaling in a PDZ domain. J Mol Biol. 2004, 335: 1105-1115. 10.1016/j.jmb.2003.11.010.

    Article  CAS  PubMed  Google Scholar 

  148. Fuentes EJ, Gilmore SA, Mauldin RV, Lee AL: Evaluation of energetic and dynamic coupling networks in a PDZ domain protein. J Mol Biol. 2006, 364: 337-351. 10.1016/j.jmb.2006.08.076.

    Article  CAS  PubMed  Google Scholar 

  149. Law AB, Fuentes EJ, Lee AL: Conservation of side-chain dynamics within a protein family. J Am Chem Soc. 2009, 131: 6322-6323. 10.1021/ja809915a.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  150. Chi CN, Elfstrom L, Shi Y, Snall T, Engstrom A, Jemth P: Reassessing a sparse energetic network within a single protein domain. Proc Natl Acad Sci USA. 2008, 105: 4679-4684. 10.1073/pnas.0711732105.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  151. Aarts M, Liu Y, Liu L, Besshoh S, Arundine M, Gurd JW, Wang YT, Salter MW, Tymianski M: Treatment of ischemic brain damage by perturbing NMDA receptor-PSD-95 protein interactions. Science. 2002, 298: 846-850. 10.1126/science.1072873.

    Article  CAS  PubMed  Google Scholar 

  152. Dev KK: Making protein interactions druggable: targeting PDZ domains. Nat Rev Drug Discov. 2004, 3: 1047-1056. 10.1038/nrd1578.

    Article  CAS  PubMed  Google Scholar 

  153. Wolde M, Fellows A, Cheng J, Kivenson A, Coutermarsh B, Talebian L, Karlson K, Piserchio A, Mierke DF, Stanton BA, Guggino WB, Madden DR: Targeting CAL as a negative regulator of DeltaF508-CFTR cell-surface expression: an RNA interference and structure-based mutagenetic approach. J Biol Chem. 2007, 282: 8099-8109. 10.1074/jbc.M611049200.

    Article  CAS  PubMed  Google Scholar 

  154. Georgescu MM, Morales FC, Molina JR, Hayashi Y: Roles of NHERF1/EBP50 in cancer. Curr Mol Med. 2008, 8: 459-468. 10.2174/156652408785748031.

    Article  CAS  PubMed  Google Scholar 

  155. Georgescu MM: NHERF1: molecular brake on the PI3K pathway in breast cancer. Breast Cancer Res. 2008, 10: 106-10.1186/bcr1992.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  156. Dev KK, Nakanishi S, Henley JM: The PDZ domain of PICK1 differentially accepts protein kinase C-alpha and GluR2 as interacting ligands. J Biol Chem. 2004, 279: 41393-41397. 10.1074/jbc.M404499200.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  157. Shan J, Shi DL, Wang J, Zheng J: Identification of a specific inhibitor of the dishevelled PDZ domain. Biochemistry. 2005, 44: 15495-15503. 10.1021/bi0512602.

    Article  CAS  PubMed  Google Scholar 

  158. Hammond MC, Harris BZ, Lim WA, Bartlett PA: Beta strand peptidomimetics as potent PDZ domain ligands. Chem Biol. 2006, 13: 1247-1251. 10.1016/j.chembiol.2006.11.010.

    Article  CAS  PubMed  Google Scholar 

  159. Fujii N, You L, Xu Z, Uematsu K, Shan J, He B, Mikami I, Edmondson LR, Neale G, Zheng J, Guy RK, Jablons DM: An antagonist of dishevelled protein-protein interaction suppresses beta-catenin-dependent tumor cell growth. Cancer Res. 2007, 67: 573-579. 10.1158/0008-5472.CAN-06-2726.

    Article  CAS  PubMed  Google Scholar 

  160. Shan J, Zheng JJ: Optimizing Dvl PDZ domain inhibitor by exploring chemical space. J Comput Aided Mol Des. 2009, 23: 37-47. 10.1007/s10822-008-9236-1.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  161. Udugamasooriya G, Saro D, Spaller MR: Bridged peptide macrocycles as ligands for PDZ domain proteins. Org Lett. 2005, 7: 1203-1206. 10.1021/ol0475966.

    Article  CAS  PubMed  Google Scholar 

  162. Udugamasooriya DG, Sharma SC, Spaller MR: A chemical library approach to organic-modified peptide ligands for PDZ domain proteins: a synthetic, thermodynamic and structural investigation. Chembiochem. 2008, 9: 1587-1589. 10.1002/cbic.200800126.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  163. Bach A, Chi CN, Olsen TB, Pedersen SW, Roder MU, Pang GF, Clausen RP, Jemth P, Stromgaard K: Modified peptides as potent inhibitors of the postsynaptic density-95/N-methyl-D-aspartate receptor interaction. J Med Chem. 2008, 51: 6450-6459. 10.1021/jm800836w.

    Article  CAS  PubMed  Google Scholar 

  164. Blazer LL, Neubig RR: Small molecule protein-protein interaction inhibitors as CNS therapeutic agents: current progress and future hurdles. Neuropsychopharmacology. 2009, 34: 126-141. 10.1038/npp.2008.151.

    Article  CAS  PubMed  Google Scholar 

  165. Houslay MD: Disrupting specific PDZ domain-mediated interactions for therapeutic benefit. Br J Pharmacol. 2009, 158: 483-485. 10.1111/j.1476-5381.2009.00359.x.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  166. Grootjans JJ, Reekmans G, Ceulemans H, David G: Syntenin-syndecan binding requires syndecan-synteny and the co-operation of both PDZ domains of syntenin. J Biol Chem. 2000, 275: 19933-19941. 10.1074/jbc.M002459200.

    Article  CAS  PubMed  Google Scholar 

  167. Raghuram V, Mak DO, Foskett JK: Regulation of cystic fibrosis transmembrane conductance regulator single-channel gating by bivalent PDZ-domain-mediated interaction. Proc Natl Acad Sci USA. 2001, 98: 1300-1305. 10.1073/pnas.031538898.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  168. Jannatipour M, Dion P, Khan S, Jindal H, Fan X, Laganiere J, Chishti AH, Rouleau GA: Schwannomin isoform-1 interacts with syntenin via PDZ domains. J Biol Chem. 2001, 276: 33093-33100. 10.1074/jbc.M105792200.

    Article  CAS  PubMed  Google Scholar 

  169. Xue Y, Ren J, Gao X, Jin C, Wen L, Yao X: GPS 2.0, a tool to predict kinase-specific phosphorylation sites in hierarchy. Mol Cell Proteomics. 2008, 7: 1598-1608. 10.1074/mcp.M700574-MCP200.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  170. Gao X, Jin C, Ren J, Yao X, Xue Y: Proteome-wide prediction of PKA phosphorylation sites in eukaryotic kingdom. Genomics. 2008, 92: 457-463. 10.1016/j.ygeno.2008.08.013.

    Article  CAS  PubMed  Google Scholar 

  171. Ballif BA, Villen J, Beausoleil SA, Schwartz D, Gygi SP: Phosphoproteomic analysis of the developing mouse brain. Mol Cell Proteomics. 2004, 3: 1093-1101. 10.1074/mcp.M400085-MCP200.

    Article  CAS  PubMed  Google Scholar 

  172. Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC, Gygi SP: Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci USA. 2004, 101: 12130-12135. 10.1073/pnas.0404720101.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  173. Schwartz D, Gygi SP: An iterative statistical approach to the identification of protein phosphorylation motifs from large-scale data sets. Nat Biotechnol. 2005, 23: 1391-1398. 10.1038/nbt1146.

    Article  CAS  PubMed  Google Scholar 

  174. Ikenoue T, Inoki K, Zhao B, Guan KL: PTEN acetylation modulates its interaction with PDZ domain. Cancer Res. 2008, 68: 6908-6912. 10.1158/0008-5472.CAN-08-1107.

    Article  CAS  PubMed  Google Scholar 

  175. Ren J, Wen L, Gao X, Jin C, Xue Y, Yao X: CSS-Palm 2.0: an updated software for palmitoylation sites prediction. Protein Eng Des Sel. 2008, 21: 639-644. 10.1093/protein/gzn039.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  176. Ren J, Gao X, Jin C, Zhu M, Wang X, Shaw A, Wen L, Yao X, Xue Y: Systematic study of protein sumoylation: Development of a site-specific predictor of SUMOsp 2.0. Proteomics. 2009, 9: 3409-3412. 10.1002/pmic.200800646.

    Article  CAS  Google Scholar 

  177. Wu H, Feng W, Chen J, Chan LN, Huang S, Zhang M: PDZ domains of Par-3 as potential phosphoinositide signaling integrators. Mol Cell. 2007, 28: 886-898. 10.1016/j.molcel.2007.10.028.

    Article  CAS  PubMed  Google Scholar 

  178. Zimmermann P, Meerschaert K, Reekmans G, Leenaerts I, Small JV, Vandekerckhove J, David G, Gettemans J: PIP(2)-PDZ domain binding controls the association of syntenin with the plasma membrane. Mol Cell. 2002, 9: 1215-1225. 10.1016/S1097-2765(02)00549-X.

    Article  CAS  PubMed  Google Scholar 

  179. Zimmermann P, Zhang Z, Degeest G, Mortier E, Leenaerts I, Coomans C, Schulz J, N'Kuli F, Courtoy PJ, David G: Syndecan recycling [corrected] is controlled by syntenin-PIP2 interaction and Arf6. Dev Cell. 2005, 9: 377-388. 10.1016/j.devcel.2005.07.011.

    Article  CAS  PubMed  Google Scholar 

  180. Mortier E, Wuytens G, Leenaerts I, Hannes F, Heung MY, Degeest G, David G, Zimmermann P: Nuclear speckles and nucleoli targeting by PIP2-PDZ domain interactions. EMBO J. 2005, 24: 2556-2565. 10.1038/sj.emboj.7600722.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  181. Zimmermann P: The prevalence and significance of PDZ domain-phosphoinositide interactions. Biochim Biophys Acta. 2006, 1761: 947-956.

    Article  CAS  PubMed  Google Scholar 

  182. Meerschaert K, Tun MP, Remue E, De GA, Boucherie C, Vanloo B, Degeest G, Vandekerckhove J, Zimmermann P, Bhardwaj N, Lu H, Cho W, Gettemans J: The PDZ2 domain of zonula occludens -1 and -2 is a phosphoinositide binding domain. Cell Mol Life Sci. 2009

    Google Scholar 

  183. Gallardo R, Ivarsson Y, Schymkowitz J, Rousseau F, Zimmermann P: Structural Diversity of PDZ-Lipid Interactions. Chembiochem. 2010, 11: 456-467. 10.1002/cbic.200900616.

    Article  CAS  PubMed  Google Scholar 

  184. Schultz J, Milpetz F, Bork P, Ponting CP: SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci USA. 1998, 95: 5857-5864. 10.1073/pnas.95.11.5857.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  185. Tochio H, Zhang Q, Mandal P, Li M, Zhang M: Solution structure of the extended neuronal nitric oxide synthase PDZ domain complexed with an associated peptide. Nat Struct Biol. 1999, 6: 417-421. 10.1038/8216.

    Article  CAS  PubMed  Google Scholar 

  186. Tochio H, Mok YK, Zhang Q, Kan HM, Bredt DS, Zhang M: Formation of nNOS/PSD-95 PDZ dimer requires a preformed beta-finger structure from the nNOS PDZ domain. J Mol Biol. 2000, 303: 359-370. 10.1006/jmbi.2000.4148.

    Article  CAS  PubMed  Google Scholar 

  187. Cohen NA, Brenman JE, Snyder SH, Bredt DS: Binding of the inward rectifier K+ channel Kir 2.3 to PSD-95 is regulated by protein kinase A phosphorylation. Neuron. 1996, 17: 759-767. 10.1016/S0896-6273(00)80207-X.

    Article  CAS  PubMed  Google Scholar 

  188. Toker A: Identifying protein kinase C substrates: an introduction. Methods Mol Biol. 2003, 233: 247-251.

    CAS  PubMed  Google Scholar 

  189. Pearson RB, Kemp BE: Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations. Methods Enzymol. 1991, 200: 62-81. full_text.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Vani Shanker and Stephan M. Feller for editing the manuscript and Thusitha Jayasundera and Kate McInrosh for reading the manuscript. This work is supported by Cancer Center Support Grant [CA21765] from the National Cancer Institute, a grant [GM081492] from the National Institutes of Health, and the American Lebanese Syrian Associated Charities (ALSAC).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jie J Zheng.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

Both authors wrote the manuscript and approved its final version.

Authors’ original submitted files for images

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Lee, HJ., Zheng, J.J. PDZ domains and their binding partners: structure, specificity, and modification. Cell Commun Signal 8, 8 (2010). https://doi.org/10.1186/1478-811X-8-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/1478-811X-8-8

Keywords