IGF-1 and latent TGF-β1 resulted in nuclear localization of β-catenin in MCF-7 cells. Nuclei are stained with DAPI (blue) while β-catenin is localized with a specific antibody (green). Treatment of MCF-7 cells with 100 nM recombinant IGF-1 + 10 nM TGF-β1 resulted in nuclear (blue) localization of β-catenin (green) (green blue overlap g-h). Treatment with vehicle control (a-b), IGF-1 (c-d) or TGF-β1 alone (e-f) showed β-catenin primarily localized at the plasma membrane. Images are representative of 3 independent experiments with consistent results.
Walsh and Damjanovski Cell Communication and Signaling 2011 9:10 doi:10.1186/1478-811X-9-10