Table 4

Cell lines with reported resistance for phosphorylation/injection of CagA ^a

Cell line

Origin

Species

H. pylori strains used

Applied methods

References

Hek293

kidney

human

P1, P12, 26695, P310

Anti-PY WB/SI

[99]

GLC4

Lung

human

P1, P12

Anti-PY WB/IPA

[74]

CHOK1

Ovary

hamster

P1, P12

Anti-PY WB/IPA

[74]

MDCK

Kidney

dog

P1, P12

Anti-PY WB/IPA

[74]

^a Abbreviations: anti-PY WB (Westernblotting of infected cell lysates using several pan-phospho-tyrosine antibodies); IPA (in vitro phosphorylation assays of CagA with cell lysates to ensure that active kinases are present in the cells of interest); SI (synchronised infection assays: bacteria were centrifuged onto Hek293 cells to ensure proper contact of bacteria with these host cells).

Backert et al. Cell Communication and Signaling 2011 9:28   doi:10.1186/1478-811X-9-28