Table 1 |
|||
|
Proteins identified in enriched secretory lysosomes from activated T cells. |
|||
|
Protein name |
Spot # |
Predicted/annotated subcellular localisation |
Predicted function |
|
|
|||
|
100 kDa coactivator |
122 |
ER,ME,PL |
biosynthesis |
|
14-3-3 protein beta |
714 |
CY,ME |
adapter |
|
14-3-3 protein epsilon |
685 |
CY,ME |
adapter |
|
14-3-3 protein zeta/delta |
708 |
CY,ME |
adapter |
|
2',3'-cyclic-nucleotide 3'-phosphodiesterase |
542 |
ME,NG |
hydrolase |
|
26S protease regulatory subunit 6A |
1037 |
CY,NU |
protein degradation |
|
26S proteasome non-ATPase regulatory subunit 2 |
165 |
ME |
proteasome |
|
3-phosphoglycerate dehydrogenase |
408 |
ME |
biosythesis |
|
acetyl-CoA acetyltransferase, cytosolic |
594 |
CY |
biosynthesis |
|
ACTB protein |
525 |
ER,ME,EX,PL,SY |
cell motility |
|
actin related protein 2 isoform b |
564 |
EN,ER,ME |
trafficking |
|
actin related protein 2/3 complex subunit 1B |
590 |
ME,PL |
trafficking |
|
actin related protein 2/3 complex subunit 2 |
674 |
EN,ME,PL,ER |
trafficking |
|
actin related protein 2/3 complex subunit 3 |
799 |
EN,ME,PL,ER |
trafficking |
|
actin related protein 2/3 complex subunit 4 |
798 |
ER,EX,ME,SY |
trafficking |
|
actin related protein 2/3 complex subunit 5-like |
804 |
ME |
trafficking |
|
actin, alpha, cardiac muscle |
570 |
ME |
cell motility |
|
actin, gamma 1 propeptide |
701 |
ME,EX,PL,SY |
cell motility |
|
actinin, alpha 4 |
144 |
ME,NU,CY |
cell motility |
|
acylamino acid-releasing enzyme |
203 |
PL, CY |
hydrolase |
|
acyl-CoA synthetase long-chain family member 4 isoform 2 |
199 |
MT,PE,ME |
metabolism |
|
adenine phosphoribosyltransferase isoform b |
784 |
ME,EX,PL |
biosynthesis |
|
adenosine deaminase |
550 |
CY,LY |
hydrolase |
|
adenylosuccinate lyase |
439 |
CY,PL |
biosynthesis |
|
adenylosuccinate synthetase |
1023 |
CY |
biosynthesis |
|
adenylyl cyclase-associated protein variant |
395 |
PL,ME |
trafficking |
|
aflatoxin aldehyde reductase AFAR |
1056 |
PL,GO |
redox protein |
|
aging-associated gene 12 |
418 |
unknown |
unclassified |
|
alanyl-tRNA synthetase |
111 |
ME,PL |
biosynthesis |
|
alcohol dehydrogenase class-3 |
587 |
PL,CY |
redox protein |
|
aldo-keto reductase family 1, member A1 |
1078 |
PL,SY |
metabolism |
|
aldolase A |
560 |
EN,ME |
metabolism |
|
aldose 1-epimerase (BLOCK25) |
596 |
CY |
metabolism |
|
aldose reductase |
981 |
ME,EX,MT |
metabolism |
|
alkyldihydroxyacetonephosphate synthase, peroxisomal |
273 |
PE |
biosynthesis |
|
alpha-tubulin |
362 |
PL |
cell motility |
|
annexin A1 |
615 |
ME,MT |
trafficking |
|
annexin A11 |
415 |
MT,EX,ME |
trafficking |
|
annexin A2 |
631 |
MT,EX,ME,secreted |
exocytosis |
|
annexin A4 |
668 |
MT,EX,ME,SY |
signal trans. |
|
annexin A5 |
660 |
MT,EX,ME,ER |
trafficking |
|
annexin A6 |
263 |
MT,EX,ME,ER |
trafficking |
|
annexin A7 isoform 2 |
504 |
EX,ME,PL |
exocytosis |
|
ARP3 actin-related protein 3 homolog |
465 |
EN,ER,ME |
trafficking |
|
ARTS-1 |
136 |
ER,ME |
immunity |
|
aryl hydrocarbon receptor interacting protein |
955 |
unknown |
unclassified |
|
asparaginyl-tRNA synthetase |
306 |
ME |
biosynthesis |
|
aspartate aminotransferase |
575 |
CY |
biosynthesis |
|
ATP citrate lyase |
96 |
ME,EX,PL |
biosynthesis |
|
ATP synthase, alpha subunit precursor |
426 |
ER,LY,NG,SY,MT |
channel |
|
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 |
413 |
LY,ME,NG,SY |
channel |
|
axin interactor, dorsalization associated protein |
632 |
unknown |
signal trans. |
|
beta adrenergic receptor kinase 1 |
220 |
CY |
GTPase |
|
bifunctional purine biosynthesis protein PURH |
326 |
ME,PL |
mutifunctional |
|
bleomycin hydrolase |
463 |
CY,PL |
hydrolase |
|
BolA-like protein 2 |
867 |
ME |
unclassified |
|
calcium binding protein 39 |
622 |
EX |
unclassified |
|
CALM3 protein |
816 |
unknown |
unclassified |
|
calreticulin precursor variant |
287 |
ER,ME,NG,EX,PL,MT |
chaperone |
|
carboxyl terminal LIM domain protein |
612 |
ER,ME,PL,EN |
cell motility |
|
catalase |
361 |
PE,ER,LY,EN,ME |
metabolism |
|
cathepsin B |
691 |
LY,ME,NG |
hydrolase |
|
cathepsin D preproprotein |
696 |
LY,ME,NG,EX,MT |
hydrolase |
|
cathepsin H |
738 |
LY |
hydrolase |
|
cathepsin S |
749 |
LY |
immunity |
|
Cbr1 In Complex With Hydroxy-Pp |
666 |
ME |
redox protein |
|
Cdc42ACK GTPASE |
790 |
ME |
cell motility |
|
centaurin beta1 |
214 |
unknown |
GTPase |
|
chaperonin (HSP60) |
338 |
ME,NG,EX,SY,MT |
chaperone |
|
chaperonin containing TCP1, subunit 2β |
424 |
ER,EN,ME,PL,MT,CY |
chaperone |
|
chaperonin containing TCP1, subunit 7η |
376 |
EN,ME,PL |
chaperone |
|
chaperonin containing TCP1, subunti 5ε |
339 |
EX,PL |
chaperone |
|
chaperonin containing TCP1, subunti 8τ |
357 |
EN,ME,EX |
chaperone |
|
chaperonin containing TCP1, subunti 8τ |
358 |
EN,ME,EX |
chaperone |
|
chromatin modifying protein 4B |
639 |
ME,EX |
trafficking |
|
chromosome 20 open reading frame 3 (BSCv) |
489 |
ME |
unclassified |
|
chromosome 9 open reading frame 19 |
831 |
EX,GO |
unclassified |
|
N2 protein |
651 |
unknown |
unclassified |
|
coactosin-like protein |
841 |
PL,SY |
unclassified |
|
cofilin 1 |
810 |
ER,ME,EX,MT |
cell motility |
|
copine I |
310 |
ME |
trafficking |
|
copine III |
340 |
ME,EX,PL |
trafficking |
|
coronin 7 |
113 |
CY,GO |
trafficking |
|
coronin, actin binding protein, 1A |
371 |
LY,PL |
cell motility |
|
coronin, actin binding protein, 1C |
353 |
ME |
multifunctional |
|
c-src tyrosine kinase |
475 |
CY,PL |
signal trans. |
|
cyclophilin A |
826 |
ME,EX,MT |
chaperone |
|
cyclophilin B |
800 |
ER,ME |
chaperone |
|
cystatin B |
857 |
ME |
protein inhibitor |
|
cysteine and glycine-rich protein 1 |
769 |
NU |
unclassified |
|
cytoskeleton associated protein |
952 |
CY |
cell motility |
|
cytosolic malate dehydrogenase |
642 |
ME,EX,PL,SY,MT |
metabolism |
|
DCHT2 Serine/threonine-protein kinase OSR1 |
332 |
ME |
signal trans. |
|
destrin isoform a |
814 |
ME,EX,MT |
cell motility |
|
differentially expressed in FDCP 6 homolog (mouse), isoform CRA_b |
228 |
unknown |
unclassified |
|
dihydropyrimidinase-like 2 |
319 |
SY |
signal trans. |
|
dimethylarginine dimethylaminohydrolase 2 |
683 |
unknown |
hydrolase |
|
dipeptidyl peptidase 4 |
81 |
ER,LY,EN,ME,EX |
hydrolase |
|
DJ-1 protein |
764 |
ME,PL,SY,MT |
redox protein |
|
DnaJ (Hsp40) homolog, subfamily A, member 1, isoform CRA_d |
499 |
ER,ME |
chaperone |
|
DnaJ (Hsp40) homolog, subfamily B, member 11 precursor |
551 |
ER |
chaperone |
|
docking protein 2 |
455 |
unknown |
unclassified |
|
dynamin 2 isoform 1 |
148 |
EN |
trafficking |
|
echinoderm microtubule associated protein like 2 variant |
929 |
CY |
cell motility |
|
EF-hand domain family, member D2 |
1012 |
unknown |
unclassified |
|
EH-domain containing 1 |
342 |
ER,LY,EN,EX,PL |
trafficking |
|
Ena-VASP-like protein |
447 |
CY |
cell motility |
|
ENC-1AS aka Beta-hexosaminidase subunit beta |
431 |
LY |
multifunctional |
|
endoplasmic reticulum protein 29 isoform 1 precursor |
721 |
ER,ME,PL |
chaperone |
|
enolase 1 variant |
496 |
ME,EX,SY,MT |
metabolism |
|
ERAP2 protein |
99 |
ER |
immunity |
|
ERBB2IP protein |
197 |
NU,CY |
multifunctional |
|
ERO1L |
311 |
ME,ER |
redox protein |
|
esterase D/formylglutathione hydrolase |
656 |
ME |
hydrolase |
|
eukaryotic translation elongation factor 1 alpha 1 |
462 |
ER,LY,EN,ME,EX,PL |
biosynthesis |
|
eukaryotic translation elongation factor 1 gamma, isoform CRA_d |
947 |
ME |
biosynthesis |
|
eukaryotic translation elongation factor 2 |
158 |
ER,EN,ME,EX |
biosynthesis |
|
eukaryotic translation initiation factor 4A |
505 |
ME |
biosynthesis |
|
eukaryotic translation initiation factor 5A |
817 |
ME |
biosynthesis |
|
extended-synaptotagmin-1 KIAA0747 protein |
155 |
ME |
unclassified |
|
ezrin |
208 |
CY |
cell motility |
|
F-actin capping protein alpha-1 subunit |
611 |
ER,EN,ME |
actin binding |
|
F-actin capping protein alpha-1 subunit variant |
623 |
ER,EN,ME |
actin binding |
|
F-actin capping protein alpha-2 subunit |
616 |
ER,EN,ME,PL |
cell motility |
|
F-actin capping protein beta subunit |
663 |
ER,EN,ME |
actin binding |
|
farnesyl pyrophosphate synthetase |
579 |
CY |
biosynthesis |
|
FK506 binding protein 1A |
856 |
ME,SY,MT |
signal trans. |
|
flotillin 1 |
486 |
LY,ME,EX |
membrane |
|
formin-binding protein 1 |
1059 |
SL, LY, CY |
adapter |
|
fructose-bisphosphate aldolase C |
565 |
ME,SY,MT |
metabolism |
|
fumarate hydratase, mitochondrial |
507 |
EN,SY,MT |
cell cycle |
|
FYN-binding protein |
71 |
CY,NU |
adapter |
|
G protein beta subunit |
638 |
ME,MT |
signal trans. |
|
galectin-1 |
851 |
ME,PL |
immunity |
|
galectin-3 |
718 |
ME,NU |
immunity |
|
gamma-enolase |
476 |
ME,PL,SY |
glycolysis |
|
gamma-glutamyl hydrolase |
629 |
LY,ME,NG,PL |
hydrolase |
|
GDP-mannose pyrophosphorylase A |
541 |
unknown |
biosythesis |
|
gelsolin-like capping protein isoform 9 |
572 |
ME,CY,NU |
cell motility |
|
GIPC1 protein |
598 |
SY,CY |
protein binding |
|
glia maturation factor gamma |
818 |
unknown |
unclassified |
|
glucosamine-6-phosphate deaminase 1 |
677 |
CY |
hydrolase |
|
glucose-6-phosphate dehydrogenase isoform b |
409 |
ME |
metabolism |
|
glucosidase II subunit beta |
126 |
ER,ME,PL |
hydrolase |
|
glucosidase, alpha; neutral AB, isoform CRA_a |
936 |
ER,ME,PL |
hydrolase |
|
glutamate carboxypeptidase |
430 |
unknown |
hydrolase |
|
glutamate Dehydrogenase-Apo Form |
437 |
ER,ME,PL,MT |
unclassified |
|
glutaredoxin 3 |
589 |
CY |
redox protein |
|
glutathione S-transferase P1 |
766 |
ER,ME,EX,PL |
metabolism |
|
glutathione synthetase |
461 |
PL |
redox protein |
|
glutathione-S-transferase kappa 1 |
765 |
PL,ME,MT,PE |
unclassified |
|
glutathione-S-transferase omega 1 |
698 |
LY,ME,NG,EX,PL,SY,MT |
metabolism |
|
glyceraldehyde-3-phosphate dehydrogenase |
610 |
LY,ME,NG,EX,PL,SY,MT |
metabolism |
|
glycyl-tRNA synthetase |
244 |
ME |
biosynthesis |
|
glyoxalase domain containing 4 |
653 |
MT |
unclassified |
|
GNAS complex locus isoform f |
531 |
EX |
multifunctional |
|
GNB1 protein |
634 |
EN,ME,EX,PL,SY |
signal trans. |
|
granzyme A |
724 |
SL |
immunity |
|
GRAP2 protein |
957 |
unknown |
unclassified |
|
GRB2 protein |
756 |
SY |
adapter |
|
GTP-binding nuclear protein Ran |
755 |
ME,EX |
trafficking |
|
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, isoform CRA_c |
582 |
EX |
GTPase |
|
guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1, isoform CRA_d |
664 |
ER |
signal trans. |
|
guanine nucleotide-binding protein G(k) subunit alpha |
585 |
ME,EX |
trafficking |
|
guanine nucleotide-binding protein subunit alpha-13 |
989 |
ME |
signal trans. |
|
haloacid dehalogenase-like hydrolase domain containing 2 |
690 |
unknown |
hydrolase |
|
heat shock 70kDa protein 1A |
278 |
ER,EN,ME,EX,MT |
chaperone |
|
heat shock 70kDa protein 5 |
226 |
ER,ME,EX,PL,MT |
chaperone |
|
heat shock 70kDa protein 8 isoform 1 |
259 |
LY,ME,NG,EX,PL,SY,MT |
chaperone |
|
heat shock 70kDa protein 8 isoform 1 |
260 |
LY,ME,NG,EX,PL,SY,MT |
chaperone |
|
heat shock protein 70 |
112 |
EX |
chaperone |
|
heat shock protein HSP 90-alpha |
969 |
ME,NG,MT |
chaperone |
|
heat shock protein HSP 90-beta |
177 |
ME,EX,MT |
chaperone |
|
hematopoietic cell-specific Lyn substrate 1 |
181 |
CY,MT |
signal trans. |
|
HEXA protein |
422 |
LY |
multifunctional |
|
hexose-6-phosphate dehydrogenase |
194 |
ER |
metabolism |
|
HIP-55 |
377 |
CY |
signal trans. |
|
histidine triad nucleotide binding protein 1 |
852 |
ME,PL,SY |
hydrolase |
|
histocompatibility (minor) HA-1 |
1072 |
unknown |
GTPase |
|
hypothetical protein |
216 |
unknown |
unclassified |
|
hypothetical protein LOC79624 |
472 |
unknown |
unclassified |
|
hypoxia up-regulated protein 1 |
47 |
ER,PL,ME |
chaperone |
|
importin subunit beta-1 |
164 |
ME |
trafficking |
|
integrin beta-2 |
80 |
PL |
membrane |
|
interleukin-16 |
210 |
secreted |
immunity |
|
isocitrate dehydrogenase 1 (NADP+), soluble, isoform CRA_b |
540 |
ME,EX,PL |
redox protein |
|
isocitrate dehydrogenase 2 (NADP+), mitochondrial, isoform CRA_b |
510 |
PL,MT |
redox protein |
|
kinase/transmembrane domain fusion protein |
1061 |
unknown |
unclassified |
|
laminin-binding protein |
543 |
ME,ER |
cell adhesion |
|
leucine aminopeptidase 3 |
432 |
CY |
protein degradation |
|
leucine rich repeat containing 57 |
747 |
unknown |
unclassified |
|
leucine-rich repeat and calponin homology domain-containing protein 5 |
908 |
MT |
protein binding |
|
leucocyte antigen CD97 |
872 |
ME,secreted |
cell adhesion |
|
leukocyte-derived arginine aminopeptidase long form variant |
102 |
unknown |
hydrolase |
|
leukotriene A4 hydrolase |
309 |
CY |
hydrolase |
|
LIM and SH3 domain protein 1 |
606 |
ER,EN,ME,PL |
adapter |
|
LIM domain-containing protein 2 |
834 |
unknown |
unclassified |
|
lin 7 homolog c |
1070 |
SY |
exocytosis |
|
L-lactate dehydrogenase |
645 |
ME,EX,SY |
metabolism |
|
L-lactate dehydrogenase B chain |
626 |
ME,EX,PL,SY,MT |
redox protein |
|
L-plastin |
266 |
CY |
actin binding |
|
L-plastin variant |
267 |
unknown |
cell motility |
|
LPXN protein |
474 |
unknown |
unclassified |
|
lymphocyte cytosolic protein 2 |
229 |
CY |
immunity |
|
lymphocyte-specific protein 1 |
959 |
PL |
immunity |
|
lysosomal acid alpha-mannosidase |
265 |
LY,ME |
hydrolase |
|
M2-type pyruvate kinase |
356 |
ME,EX,SY |
metabolism |
|
Macrophage Migration Inhibitory Factor (Mif) With Hydroxphenylpyruvate |
862 |
ME,EX,PL,SY |
immunity |
|
MAGUK p55 subfamily member 7 |
292 |
PL |
protein binding |
|
methylenetetrahydrofolate dehydrogenase 1 |
139 |
EN,ME,PL,MT |
multifunctional |
|
methylthioadenosine phosphorylase |
697 |
CY |
metabolism |
|
MHC class I antigen |
533 |
ME |
immunity |
|
MHC class I antigen |
865 |
ME |
immunity |
|
MHC class II antigen |
953 |
ME |
immunity |
|
MHC class II antigen DR alpha chain |
1050 |
LY |
immunity |
|
MHC class II antigen DR52 |
1083 |
ME |
immunity |
|
microtubule-associated protein, RP/EB family, member 1 |
665 |
ME,PL |
cell motility |
|
mitochondrial ATP synthase, H+ transporting F1 complex beta subunit |
443 |
MT |
trafficking |
|
mitochondrial trifunctional protein, alpha subunit precursor |
253 |
PL, MT |
metabolism |
|
mitogen-activated protein kinase 1 |
569 |
ME,PL |
signal trans. |
|
mitogen-activated protein kinase kinase 1 interacting protein 1 |
943 |
LY |
adapter |
|
mitogen-activated protein kinase kinase 2 |
509 |
unknown |
signal trans. |
|
moesin, isoform CRA_b |
246 |
EN,ME,EX,PL,MT |
cell motility |
|
mps one binder kinase activator-like 1B |
758 |
unknown |
unclassified |
|
myosin IG |
75 |
unknown |
trafficking |
|
myosin light polypeptide 6 |
830 |
ME |
cell motility |
|
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75 kDa (NADH-coenzyme Q reductase) |
896 |
ER,ME,MT |
trafficking |
|
NCK adaptor protein 1 |
506 |
CY,ER |
adapter |
|
NECAP endocytosis associated 2 |
1010 |
EN |
trafficking |
|
NESH protein |
434 |
unknown |
unclassified |
|
N-ethylmaleimide-sensitive factor attachment protein, alpha |
652 |
ME,NG,PL |
trafficking |
|
neuroblastoma RAS viral (v-ras) oncogene homolog |
779 |
GO,CY |
trafficking |
|
neuropolypeptide h3 |
781 |
ME,EX,SY |
protein inhibitor |
|
neutrophil adherence receptor alpha-M subunit |
36 |
membrane |
cell adhesion |
|
niban protein isoform 2 |
38 |
CY |
signal trans. |
|
NME1-NME2 protein |
823 |
CY,NU |
multifunctional |
|
nuclear chloride channel |
684 |
ME,EX,PL,MT |
channel |
|
nucleobindin 1 variant |
335 |
unknown |
unclassified |
|
nucleoside phosphorylase |
670 |
CY,PL |
cell cycle |
|
nucleosome assembly protein 1-like 1, isoform CRA_d |
315 |
ME,PL,NU |
cell cycle |
|
Obg-like ATPase 1 |
511 |
EN,ME,PL |
hydrolase |
|
otubain 1 |
637 |
ME |
hydrolase |
|
PA2G4 protein |
490 |
unknown |
unclassified |
|
PDCD6IP protein |
171 |
unknown |
unclassified |
|
perforin-1 |
280 |
SL |
immunity |
|
peroxiredoxin 1 |
774 |
ER,LY,EN,ME,NG,PL,MT |
redox protein |
|
peroxiredoxin 2 |
778 |
ER,EN,ME,SY,MT |
redox protein |
|
peroxiredoxin 3 |
768 |
ME,PL,MT |
redox protein |
|
peroxiredoxin 4 |
737 |
ER,EN,ME |
redox protein |
|
peroxiredoxin 6 |
945 |
LY,ME,EX,PL,SY |
redox protein |
|
PGAM1 |
730 |
ME,EX,SY |
metabolism |
|
PHB |
948 |
unknown |
unclassified |
|
phosphatase 2a |
316 |
MT |
multifunctional |
|
phosphatidylinositol-5-phosphate 4-kinase, type II, alpha |
457 |
NG,PL |
metabolism |
|
phosphofructokinase, liver |
939 |
unknown |
glycolysis |
|
phosphofructokinase, platelet |
196 |
ME,PL |
glycolysis |
|
phosphoglucose isomerase |
390 |
ME,EX,PL,MT |
multifunctional |
|
phosphoglycerate kinase 1 |
537 |
ME,EX,SY,MT |
metabolism |
|
phospholipase C, delta 1 variant |
178 |
unknown |
signal trans. |
|
phosphoribosyl pyrophosphate synthetase 1 variant |
1080 |
unknown |
biosynthesis |
|
phosphoribosylaminoimidazole carboxylase, - succinocarboxamide synthetase, isoform CRA_b |
523 |
EN,SY |
multifunctional |
|
phosphoribosylformylglycinamidine synthase |
64 |
CY |
biosynthesis |
|
phosphoserine aminotransferase 1 |
988 |
ME |
biosynthesis |
|
phostensin |
91 |
CY |
unclassified |
|
poly(A) binding protein, cytoplasmic 1, isoform CRA_c |
256 |
ER,EN,ME,PL |
metabolism |
|
poly(rC) binding protein 1 |
1082 |
ME,CY,NU |
unclassified |
|
potassium voltage-gated channel, shaker-related subfamily, beta member 2 isoform 2 |
635 |
CY |
channel |
|
PPP5C protein |
364 |
CY,NU |
hydrolase |
|
profilin-1 |
848 |
ME,EX,PL,MT |
actin binding |
|
programmed cell death protein 10 |
741 |
unknown |
apoptosis |
|
proline synthetase co-transcribed homolog |
699 |
CY |
unclassified |
|
prolyl 4-hydroxylase, alpha subunit |
337 |
ER,ME |
redox protein |
|
prolyl 4-hydroxylase, beta subunit precursor |
348 |
ER,ME,EX,PL,MT |
redox protein |
|
prolyl endopeptidase |
234 |
CY |
protein degradation |
|
proteasome (prosome, macropain) subunit, alpha type, 7(PSMA7) |
729 |
CY,Proteasom |
hydrolase |
|
proteasome 26S non-ATPase subunit 13 isoform 1 |
577 |
ME |
proteasome |
|
proteasome 26S subunit, ATPase, 2 |
498 |
CY,NU |
unclassified |
|
proteasome 26S subunit, ATPase, 5 |
514 |
CY,NU |
unclassified |
|
proteasome activator complex subunit 1 isoform 1 |
703 |
PL,MT |
immunity |
|
proteasome activator complex subunit 2 |
689 |
ME |
immunity |
|
proteasome alpha 2 subunit variant |
754 |
CY |
hydrolase |
|
proteasome subunit, alpha type, 1 |
687 |
ME |
hydrolase |
|
proteasome subunit, alpha type, 5 |
1009 |
ME |
hydrolase |
|
proteasome subunit, alpha type, 6 |
734 |
CY,NU |
hydrolase |
|
proteasome subunit, beta type, 1 |
750 |
ME,CY |
hydrolase |
|
proteasome subunit, beta type, 2 |
780 |
CY,NU |
hydrolase |
|
proteasome subunit, beta type, 4 |
944 |
CY,NU |
hydrolase |
|
proteasome subunit, beta type, 8 |
773 |
PL,CY,NU |
immunity |
|
protein ARMET |
805 |
ME, secreted |
unclassified |
|
protein diaphanous homolog 1 |
45 |
ME |
cell motility |
|
protein disulfide isomerase-associated 4 |
1060 |
ER,ME,PL |
chaperone |
|
protein disulfide isomerase-related protein 5 |
458 |
ER,ME |
chaperone |
|
protein disulfide-isomerase A3 |
379 |
ER,LY,ME,NG,EX,PL |
chaperone |
|
protein phosphatase 1, catalytic subunit, alpha isoform 1 |
603 |
EX |
hydrolase |
|
protein phosphatase 1, catalytic subunit, beta isoform |
617 |
ME,PL |
hydrolase |
|
protein tyrosine phosphatase 1b |
536 |
ME,ER |
hydrolase |
|
protein tyrosine phosphatase, non-receptor type 6 isoform 1 variant |
317 |
unknown |
hydrolase |
|
protein-tyrosine kinase fyn isoform c |
373 |
EN,CY |
signal trans. |
|
PYD and CARD domain containing |
771 |
CY |
apoptosis |
|
pyrophosphatase 1 |
654 |
ME,MT |
hydrolase |
|
pyruvate kinase 3 isoform 2 |
346 |
ME,EX,SY |
metabolism |
|
R33729_1 (Interleukin-25) |
837 |
ME,secreted |
signal trans. |
|
Rab GDP dissociation inhibitor beta |
469 |
ME,EX,PL,MT |
GTPase |
|
raftlin cell migration-inducing gene 2 |
193 |
PL |
unclassified |
|
Rap1a |
785 |
EN,ME,MT |
GTPase |
|
Rap1-GTP-interacting adapter molecule |
141 |
CY |
signal trans. |
|
Ras GTPase-activating-like protein IQGAP2 |
1069a |
EN |
signal trans. |
|
related RAS viral (r-ras) oncogene homolog 2 isoform a |
1049 |
LY,ME,EX |
GTPase |
|
Rho GDP dissociation inhibitor (GDI) alpha |
716 |
ME,PL,MT |
GTPase |
|
Rho GDP dissociation inhibitor (GDI) beta |
728 |
CY |
GTPase |
|
Rho GTPase activating protein 1 |
441 |
PL |
GTPase |
|
Rho GTPase-activating protein 9 |
998 |
unknown |
GTPase |
|
ribosomal protein L11 |
797 |
ribosome |
biosynthesis |
|
ribosomal protein L12 |
809 |
EN,ribosom |
biosynthesis |
|
S-adenosylhomocysteine hydrolase |
513 |
ME |
hydrolase |
|
Sec23 homolog A |
221 |
ER,ME,PL |
trafficking |
|
Sec23B protein |
1001 |
EN |
trafficking |
|
septin 2 |
554 |
ME,EX,SY |
unclassified |
|
septin 7 |
484 |
ME,PL,SY |
unclassified |
|
septin-9 delta |
558 |
ME |
unclassified |
|
septin-9 gamma |
973 |
ME |
unclassified |
|
serine/threonine phosphatase 1 gamma |
985 |
MT,SY |
hydrolase |
|
serine/threonine-protein kinase PAK 2 |
352 |
PL |
signal trans. |
|
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
621 |
MT |
signal trans. |
|
serine/threonine-protein phosphatase 2A regulatory subunit B |
592 |
NU |
signal trans. |
|
serpin peptidase inhibitor,clade B,member 1 |
548 |
CY |
protein inhibitor |
|
seryl-tRNA synthetase |
365 |
ME,PL |
tRNA processing |
|
SH2 domain protein 1A |
840 |
CY |
signal trans. |
|
SH3-containing protein, Endophilin-B1 |
1081 |
CY,GO,MT |
apoptosis |
|
SHUJUN-1 |
795 |
CY |
cell motility |
|
signal transducer and activator of transcription 1, 91kDa, isoform CRA_d |
188 |
CY,NU |
signal trans. |
|
similar to metallo-beta-lactamase superfamily protein |
686 |
unknown |
hydrolase |
|
small GTP binding protein Rac2, isoform CRA_c |
1006 |
unknown |
signal trans. |
|
soc-2 suppressor of clear homolog |
318 |
CY |
unclassified |
|
solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1 |
460 |
ME,EX,PL |
scaffolding |
|
sorting nexin 17 |
421 |
EN,ME,PL |
trafficking |
|
sorting nexin 6 |
466 |
CY |
trafficking |
|
src kinase associated phosphoprotein 1 isoform 1 |
417 |
CY,NU |
signal trans. |
|
stathmin 1/oncoprotein 18 |
820 |
SY |
cell motility |
|
stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein) |
323a |
ME,PL,SY |
chaperone |
|
stromal cell-derived factor 2-like 1 precursor |
767 |
ER |
unclassified |
|
superoxide dismutase 1, soluble |
806 |
ME,EX,MT |
redox protein |
|
syntaxin binding protein 1 |
305 |
ME,NG,EX,PL,SY |
trafficking |
|
syntaxin binding protein 2 |
302 |
EX,PL |
trafficking |
|
syntaxin binding protein 3 variant |
294 |
ME,PL |
trafficking |
|
talin-1 |
920 |
EN,ME,PL |
cell motility |
|
tapasin isoform 3 precursor |
495 |
ER,ME |
immunity |
|
TC4 protein |
736 |
NU |
GTPase |
|
T-complex polypeptide 1 |
1030 |
ER,EN,ME,EX |
chaperone |
|
T-complex protein 1 subunit gamma |
307 |
CY |
chaperone |
|
testin isoform 1 |
456 |
unknown |
unclassified |
|
thioredoxin domain-containing protein 4 precursor |
502 |
ER,ME,PL |
scaffolding |
|
transfer RNA-Trp synthetase |
411 |
ME,PL |
biosynthesis |
|
transgelin-2 |
787 |
ME,MT |
unclassified |
|
transketolase |
276 |
ME,EN |
unclassified |
|
translocon-associated protein subunit delta |
811 |
ME,ER |
trafficking |
|
triosephosphate isomerase 1 |
742 |
ME,EX,SY,MT |
unclassified |
|
tripeptidyl-peptidase 1 |
992 |
LY,ME,NG,PL,MT |
protein degradation |
|
tropomodulin 3 |
561 |
ER,ME |
cell motility |
|
tropomyosin 3 isoform 2 |
676 |
unknown |
unclassified |
|
tropomyosin 4 |
672 |
ME |
unclassified |
|
Tu translation elongation factor, mitochondrial |
517 |
LY,ME,PL,MT |
biosynthesis |
|
tubulin alpha 6 variant |
363 |
ME,PL |
cell motility |
|
tubulin tyrosine ligase-like family, member 12 |
897 |
ME |
trafficking |
|
tubulin, beta |
407 |
ME,PL,SY |
cell motility |
|
tubulin, beta polypeptide |
433 |
ME,PL,SY |
cell motility |
|
tumor rejection antigen (gp96) 1 |
118 |
ER,ME,PL,GO |
chaperone |
|
tumor susceptibility gene 101 |
470 |
EX |
trafficking |
|
twinfilin-like protein |
578 |
CY |
cell motility |
|
tyrosine kinase LCK |
399 |
CY |
signal trans. |
|
tyrosine-protein phosphatase non-receptor type 6 |
325 |
CY,NU |
signal trans. |
|
tyrosyl-tRNA synthetase |
366 |
ME,PL |
signal trans. |
|
ubiquitin associated and SH3 domain containing protein A |
913 |
CY,NU |
protein degradation |
|
ubiquitin specific peptidase 5 isoform 2 |
154 |
LY,ME,NG |
protein degradation |
|
ubiquitin specific protease 14 isoform a |
344 |
PL |
protein degradation |
|
ubiquitin-conjugating enzyme E2 L3 |
882 |
MT |
protein degradation |
|
ubiquitin-conjugating enzyme E2 N |
839 |
ME,EX,MT |
differentiation |
|
ubiquitin-like modifier-activating enzyme 1 |
120 |
MT,ME |
protein degradation |
|
UDP-glucose ceramide glucosyltransferase-like 1 isoform 1 |
39 |
ER,ME |
chaperone |
|
UDP-glucose pyrophosphorylase 2 isoform b |
442 |
EN,ME |
metabolism |
|
UNC-112 related protein 2 long form |
971 |
PL |
cell adhesion |
|
unnamed protein product |
706 |
unknown |
unclassified |
|
UPF0550 protein C7orf28 |
450 |
ME |
unclassified |
|
vacuolar H+-ATPase 56,000 subunit |
414 |
LY,ME,NG,SY |
channel |
|
vacuolar protein sorting 45A |
322 |
LY,EN |
trafficking |
|
vacuolar sorting protein 33A |
1067 |
EN,LY |
trafficking |
|
valosin-containing protein |
159 |
unknown |
unclassified |
|
vasodilator-stimulated phosphoprotein |
503 |
PL |
cell motility |
|
vinculin |
108 |
ME |
cell motility |
|
voltage-dependent anion channel 1 |
658 |
ER,LY,ME,NG,EX,PL,SY,MT |
channel |
|
voltage-dependent anion channel 2 |
657 |
SY,MT |
channel |
|
voltage-dependent anion channel 3 |
688 |
EN,ME,MT |
channel |
|
V-type proton ATPase subunit d 1 |
599 |
LY,EN,ME,SY |
channel |
|
WD repeat domain 1 |
304 |
EN,ME,EX |
cell motility |
|
Wiskott-Aldrich syndrome protein |
323b |
CY |
cell motility |
|
XRP2 protein |
546 |
ME |
signal trans. |
|
zeta-chain associated protein kinase 70kDa |
277 |
CY |
signal trans. |
|
|
|||
|
397 individual proteins were identified to be associated with enriched secretory lysosomes from human T cell blasts. The proteins are listed by name, followed by individual spot numbers and the predicted/annotated subcellular localisation and function. Abbreviations: LY: lysosomes, ME: melanosomes, PL: platelet granules, SY: synaptosomes, EX: exosomes, CG: cytotoxic granules, NG: neuromelanin granules, EN: endosomes, MT: mitochondria, GO: Golgi, PE: peroxisomes, CY: cytoplasm, ER: endoplasmic reticulum and NU: nucleus. For detailed information on individual spots/proteins, please refer to the additional files. |
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|
Schmidt et al. Cell Communication and Signaling 2011 9:4 doi:10.1186/1478-811X-9-4 |
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